Evidence map›Paper›PMID 30042427›Full record

ArticleNature communications2018

Abp1 promotes Arp2/3 complex-dependent actin nucleation and stabilizes branch junctions by antagonizing GMF.

Siyang Guo, Olga S Sokolova, Johnson Chung, Shae Padrick, Jeff Gelles, Bruce L Goode

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Mechanisms of actin disassembly and turnover.The Journal of cell biology · 2023
    Review
  8. Article
  9. Article
  10. Article
  11. Single-molecule analysis of actin filament debranching by cofilin and GMF.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  12. Emerging Concepts in Defective Macrophage Phagocytosis in Cystic Fibrosis.International journal of molecular sciences · 2022
    Review
  13. Structure of Arp2/3 complex at a branched actin filament junction resolved by single-particle cryo-electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  14. Load adaptation by endocytic actin networks.Molecular biology of the cell · 2022
    Article
  15. Review
  16. Review
  17. Review
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Siyang GuoDepartment of Biology, Brandeis University, Waltham, MA, 02454, USA.ORCID http://orcid.org/0000-0003-2352-829X
Olga S SokolovaDepartment of Biology, Moscow M.V. Lomonosov University, Moscow, 119234, Russia.
Johnson ChungDepartment of Biochemistry, Brandeis University, Waltham, MA, 02454, USA.
Shae PadrickDepartment of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, 19102, USA.
Jeff GellesDepartment of Biochemistry, Brandeis University, Waltham, MA, 02454, USA.ORCID http://orcid.org/0000-0001-7910-3421
Bruce L GoodeDepartment of Biology, Brandeis University, Waltham, MA, 02454, USA. goode@brandeis.edu.
Brandeis University · USDrexel University · USLomonosov Moscow State University · RU

Funding

MECHNISMS REGULATING ACTIN ASSEMBLYR01GM063691 · NIGMS · BRANDEIS UNIVERSITY · PI GOODE, BRUCE L · 2002 to 2019
$7.5M
Molecular Mechanisms coordinating the actin and microtubule cytoskeletonsR01GM098143 · NIGMS · BRANDEIS UNIVERSITY · PI GELLES, JEFF, GOODE, BRUCE L · 2012 to 2019
$2.6M
National Science Foundation (NSF) MRSEC DMR-1420382NIGMS NIH HHS R01 GM063691NIGMS NIH HHS R01 GM098143Office of Extramural Research, National Institutes of Health (OER) RO1 GM063691Russian Science Foundation (RSF) 14-14-00234
6 · The paper itself

Abstract

Formation and turnover of branched actin networks underlies cell migration and other essential force-driven processes. Type I nucleation-promoting factors (NPFs) such as WASP recruit actin monomers to Arp2/3 complex to stimulate nucleation. In contrast, mechanisms of type II NPFs such as Abp1 (also known as HIP55 and Drebrin-like protein) are less well understood. Here, we use single-molecule analysis to investigate yeast Abp1 effects on Arp2/3 complex, and find that Abp1 strongly enhances Arp2/3-dependent branch nucleation by stabilizing Arp2/3 on sides of mother filaments. Abp1 binds dynamically to filament sides, with sub-second lifetimes, yet associates stably with branch junctions. Further, we uncover a role for Abp1 in protecting filament junctions from GMF-induced debranching by competing with GMF for Arp2/3 binding. These data, combined with EM structures of Abp1 dimers bound to Arp2/3 complex in two different conformations, expand our mechanistic understanding of type II NPFs.

Indexed as

Actin CytoskeletonActin-Related Protein 2-3 ComplexActinsGlia Maturation FactorMicrofilament ProteinsMicroscopy, Electron, TransmissionModels, MolecularMultiprotein ComplexesProtein BindingProtein ConformationProtein MultimerizationSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsABP1 protein, S cerevisiaeActin-Related Protein 2-3 ComplexActinsGlia Maturation FactorMicrofilament ProteinsMultiprotein ComplexesSaccharomyces cerevisiae Proteins

Identifiers

PMID30042427
PMCPMC6057921
OpenAlexW2884617690

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.