ArticleThe Journal of general virology2018
Modification of betanodavirus virulence by substitutions in the 3' terminal region of RNA2.
Article in The Journal of general virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Giant grouper nervous necrosis virus subgenomic RNA3 is transcribed by a premature termination mechanism via intragenomic RNA-RNA pairing.Virology journal · 2025Article
- Genomic Differences Between Nervous Necrosis Virus (NNV) Reassortants Isolated From Wild and Farmed Fish: Implications for Viral Fitness, Temperature Adaptation and Virulence.Transboundary and emerging diseases · 2025Article
- A Potential Nervous Necrosis Virus (NNV) Live Vaccine for Sole Obtained by Genomic Modification.Animals : an open access journal from MDPI · 2024Article
- Cells and Fugu Response to Capsid of BFNNV Genotype.Viruses · 2023Article
- The genetic variability and evolution of red-spotted grouper nervous necrosis virus quasispecies can be associated with its virulence.Frontiers in microbiology · 2023Article
- Characterization of Nervous Necrosis Virus (NNV) Nonstructural Protein B2 and Its Enhancement on Virus Proliferation.Viruses · 2022Article
- Multiple isoforms of HSP70 and HSP90 required for betanodavirus multiplication in medaka cells.Archives of virology · 2022Article
- Pathogenicity of Different Betanodavirus RGNNV/SJNNV Reassortant Strains in European Sea Bass.Pathogens (Basel, Switzerland) · 2022Article
- Differential Nervous Necrosis Virus (NNV) Replication in Five Putative Susceptible Cell Lines.Pathogens (Basel, Switzerland) · 2021Article
- Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence.Pathogens (Basel, Switzerland) · 2021Article
- Betanodavirus and VER Disease: A 30-year Research Review.Pathogens (Basel, Switzerland) · 2020Review
- Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase.Veterinary research · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Betanodaviruses have bi-segmented positive-sense RNA genomes, consisting of RNAs 1 and 2. For some members of the related genus alphanodavirus, the 3' terminal 50 nucleotides (nt) of RNA2, including a predicted stem-loop structure (3'SL), are essential for replication. We investigate the possible existence and role of a similar structure in a reassortant betanodavirus strain (RGNNV/SJNNV). In this study, we developed three recombinant strains containing nucleotide changes at positions 1408 and 1412. Predictive models showed stem-loop structures involving nt 1398-1421 of the natural reassortant whereas this structure is modified in the recombinant viruses harbouring point mutations r1408 and r1408-1412, but not in r1412. Results obtained from infectivity assays showed differences between the reference strains and the mutants in both RNA1 and RNA2 synthesis. Moreover, an imbalance between the synthesis of both segments was demonstrated, mainly with the double mutant. All these results suggest an interaction between RNA1 and the 3' non-coding regions (3'NCR) of RNA2. In addition, the significant attenuation of the virulence for Senegalese sole and the delayed replication of r1408-1412 in brain tissues may point to an interaction of RNA2 with host cellular proteins.
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