ArticleCurrent protocols in toxicology2018
Newly Revised Quantitative PCR-Based Assay for Mitochondrial and Nuclear DNA Damage.
Article in Current protocols in toxicology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Klotho Is Cardioprotective in the mdx Mouse Model of Duchenne Muscular Dystrophy.The American journal of pathology · 2025Article
- A blood-based marker of mitochondrial DNA damage in Parkinson's disease.Science translational medicine · 2023Article
- Spatial mapping of the DNA adducts in cancer.DNA repair · 2023Review
- Radioprotective and Radiomitigative Effects of Melatonin in Tissues with Different Proliferative Activity.Antioxidants (Basel, Switzerland) · 2021Article
- Analysis of the mechanism of damage produced by thiazole orange photoinactivation in apheresis platelets.Blood transfusion = Trasfusione del sangue · 2021Article
- Single-Cell Approaches for Studying the Role of Mitochondrial DNA in Neurodegenerative Disease.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Mitochondrial DNA damage as a potential biomarker of LRRK2 kinase activity in LRRK2 Parkinson's disease.Scientific reports · 2020Article
- Assessment of Nuclear and Mitochondrial DNA, Expression of Mitochondria-Related Genes in Different Brain Regions in Rats after Whole-Body X-ray Irradiation.International journal of molecular sciences · 2020Article
- A higher throughput assay for quantification of melphalan-induced DNA damage in peripheral blood mononuclear cells.Scientific reports · 2019Article
- PINK1 attenuates mtDNA release in alveolar epithelial cells and TLR9 mediated profibrotic responses.PloS one · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Given the crucial role of DNA damage in human health and disease, it is important to be able to accurately measure both mitochondrial and nuclear DNA damage. This article describes a method based on a long-amplicon quantitative PCR-based assay that does not require a separate mitochondrial isolation step, which can often be labor-intensive and generate artifacts. The detailed basic protocol presented here is newly revised, with particular attention to application in Homo sapiens, Rattus norvegicus, and Caenorhabditis elegans resulting from changes in availability of PCR reagents. Optimized extraction support protocols are also described for high-quality DNA from multiple rat tissues for which these procedures had not previously been described. © 2018 by John Wiley & Sons, Inc.
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