ArticleCurrent protocols in mouse biology2018
Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease.
Article in Current protocols in mouse biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Article
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- A stress-dependent TDP-43 SUMOylation program preserves neuronal function.Molecular neurodegeneration · 2025Article
- Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines.Scientific reports · 2024Article
- The development and characterization of a CRISPR/Cas9-mediated PD-1 functional knockout rat as a tool to study idiosyncratic drug reactions.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Genetic and Molecular Quality Control of Genetically Engineered Mice.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Laforin targets malin to glycogen in Lafora progressive myoclonus epilepsy.Disease models & mechanisms · 2023Article
- Acetylation of PAX7 controls muscle stem cell self-renewal and differentiation potential in mice.Nature communications · 2021Article
- Targeted genome editing in vivo corrects a Dmd duplication restoring wild-type dystrophin expression.EMBO molecular medicine · 2021Article
- Cancers from NovelCancer research · 2020Article
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- A novel mouse model of Duchenne muscular dystrophy carrying a multi-exonicDisease models & mechanisms · 2020Article
- Article
- Principles of Genetic Engineering.Genes · 2020Review
- Control of homologous recombination by the HROB-MCM8-MCM9 pathway.Genes & development · 2019Article
- Off- and on-target effects of genome editing in mouse embryos.The Journal of reproduction and development · 2019Article
- Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation.The Journal of biological chemistryArticle
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2 authors at 2 institutions in 1 country.
Funding
Abstract
Mice carrying patient-associated point mutations are powerful tools to define the causality of single-nucleotide variants to disease states. Epitope tags enable immuno-based studies of genes for which no antibodies are available. These alleles enable detailed and precise developmental, mechanistic, and translational research. The first step in generating these alleles is to identify within the target sequence-the orthologous sequence for point mutations or the N or C terminus for epitope tags-appropriate Cas9 protospacer sequences. Subsequent steps include design and acquisition of a single-stranded oligonucleotide repair template, synthesis of a single guide RNA (sgRNA), collection of zygotes, and microinjection or electroporation of zygotes with Cas9 mRNA or protein, sgRNA, and repair template followed by screening of born mice for the presence of the desired sequence change. Quality control of mouse lines includes screening for random or multicopy insertions of the repair template and, depending on sgRNA sequence, off-target mutations introduced by Cas9. © 2018 by John Wiley & Sons, Inc.
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