ArticleCurrent protocols in cytometry2018
A Practical Cryopreservation and Staining Protocol for Immunophenotyping in Population Studies.
Article in Current protocols in cytometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- A Translational Randomized Trial of Perioperative Arginine Immunonutrition on Natural Killer Cell Function in Colorectal Cancer Surgery Patients.Annals of surgical oncology · 2022Trial
- Associations between immunosenescence and domain-specific cognition in the Health and Retirement Study Harmonized Cognitive Assessment Protocol.BMC biology · 2026Article
- Peripheral immunosenescence biomarkers and longitudinal cognitive decline: a large population-based study.npj aging · 2026Article
- Immune cell subsets linking insomnia trajectories and incident cognitive impairment: a prospective cohort study.BMC medicine · 2026Article
- Integration of multiomic and multi-phenotypic data identifies biological pathways associated with physical fitness.Communications biology · 2026Article
- Adaptive immune cells and kidney function in older adults: sex-specific associations in the health and retirement study (HRS).Frontiers in aging · 2026Article
- "Recommendations for clinical study protocols for immune and inflammatory profiling in Parkinson's disease".NPJ Parkinson's disease · 2025Review
- Assessment of immunotoxicity in the 21st century: Where we are and what we need to replace animals.Current opinion in toxicology · 2025Article
- The aging immune system and all-cause mortality in older americans: differences across sex and race/ethnicity.Immunity & ageing : I & A · 2025Article
- A 3D Co-Culture Scaffold Approach to Assess Spatially Fractionated Radiotherapy Bystander and Abscopal Immune Effects on Clonogenic Survival.International journal of molecular sciences · 2025Article
- A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM).Immunological investigations · 2024Article
- TGF-β neutralization attenuates tumor residency of activated T cells to enhance systemic immunity in mice.iScience · 2024Article
- Distinctive CD56NPJ Parkinson's disease · 2024Article
- Technical pitfalls when collecting, cryopreserving, thawing, and stimulating human T-cells.Frontiers in immunology · 2024Review
- Enhanced fetal hematopoiesis in response to symptomatic SARS-CoV-2 infection during pregnancy.Communications medicine · 2023Article
- Cohabitation as a determinant of adaptive and innate immune cell profiles: Findings from the Health and Retirement Study.Brain, behavior, & immunity - health · 2023Article
- Age-related Differences in T-cell Subsets and Markers of Subclinical Inflammation in Aging Are Independently Associated With Type 2 Diabetes in the Health and Retirement Study.Canadian journal of diabetes · 2023Article
- Epigenetic-based age acceleration in a representative sample of older Americans: Associations with aging-related morbidity and mortality.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Effect of Renal Denervation on T Cells in Patients with Resistant Hypertension.International journal of molecular sciences · 2023Article
- Immune cells are associated with mortality: the Health and Retirement Study.Frontiers in immunology · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Large population-based cohort studies, through their prospective collection of a broad range of health information, represent an invaluable resource for novel insights into the pathogenesis of human diseases. Collection and cryopreservation of viable cells from blood samples is becoming increasingly common in large cohorts as these cells are a valuable resource for immunophenotyping and functional studies. The cryopreservation of peripheral blood mononuclear cells (PBMCs), thawing, and immunophenotyping protocols used to immunophenotype 9938 participants in the Health and Retirement Study (HRS) are described. The extensive quality control involved in a large-scale immunophenotyping epidemiological study is also outlined. The existing literature on the effect of cryopreservation on various immune cell subsets including T, B, NK cells, monocytes, and dendritic cells is provided. © 2018 by John Wiley & Sons, Inc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.