ArticleOdontology2019
Evaluation of the biocompatibility of root canal sealers on human periodontal ligament cells ex vivo.
Article in Odontology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 52 citations in OpenAlex.
- Antimicrobial additives in resin-based endodontic sealers: a scoping review of antimicrobial efficacy, physicochemical properties, and cytotoxicity.Frontiers in dental medicine · 2026Pooled it
- Endodontic sealers based on calcium silicates: a systematic review.Odontology · 2019Pooled it
- Effect of root canal moisture on intratubular penetration of bioceramic sealer using two obturation techniques: A confocal laser scanning microscopic study.Journal of conservative dentistry and endodontics · 2026Article
- Evaluation of tissue response of experimental calcium silicate-based endodontic sealers: An in vivo study.European journal of oral sciences · 2026Article
- Cytotoxicity of Root Canal Sealers and Potential Clinical Implications: A Comprehensive Systematic Review of In Vitro Studies.Journal of clinical medicine · 2026Review
- Cytotoxicity and genotoxicity evaluation of AH Plus, Well-Root ST, and AH Plus Bioceramic Sealer.BMC oral health · 2025Article
- Evaluation of the Genotoxicity and Cytotoxicity of Bioceramic Endodontic Sealers in HepG2 and V79 Cell Lines: An In Vitro Study Using the Comet and Micronucleus Assays.Journal of functional biomaterials · 2025Article
- Comparative analysis of the inflammatory response of human gingival fibroblasts to NeoSEALER Flo and CeraSeal bioceramic sealers: an in vitro study.BMC oral health · 2025Article
- Biocompatibility and bioactivity of bioceramic endodontic sealer: NeoSealer Flo.Journal of applied oral science : revista FOB · 2025Article
- Comparison of biocompatibility of epoxy resin and bioceramic-based root canal sealers.Clinical oral investigations · 2024Article
- A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer.Clinical oral investigations · 2024Article
- Cytotoxicity and genotoxicity of bioceramic root canal sealers compared to conventional resin-based sealer.Scientific reports · 2024Article
- Real-time evaluation of the biocompatibility of calcium silicate-based endodontic cements: An in vitro study.Clinical and experimental dental research · 2023Article
- Effect of Different Sealers on the Cytocompatibility and Osteogenic Potential of Human Periodontal Ligament Stem Cells: An In Vitro Study.Journal of clinical medicine · 2023Article
- Cytotoxicity comparison of Bio C Sealer against multiple root canal sealers.Journal of clinical and experimental dentistry · 2023Article
- Evaluation of Bond Strength of Four Different Root Canal Sealers.Materials (Basel, Switzerland) · 2022Article
- The dentine-sealer interface: Modulation of antimicrobial effects by irrigation.International endodontic journal · 2022Article
- Biocompatibility of an experimental endodontic sealer (Resil) in comparison with AH26 and AH-Plus in rats: An animal study.Journal of dental research, dental clinics, dental prospects · 2022Article
- MTA-Based Cements: Biocompatibility and Effects on the Gene Expression of Collagen Type 1 and TGF-BioMed research international · 2022Article
- Calcium Silicate-Based Root Canal Sealers: A Narrative Review and Clinical Perspectives.Materials (Basel, Switzerland) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to evaluate the biocompatibility of two comparatively new calcium silicate containing sealers (MTA-Fillapex and BioRoot-RCS) with that of two established sealers (AH-Plus, epoxy resin-based; Pulp-Canal-Sealer, zinc oxide eugenol containing). Human periodontal ligament cells (PDL-cells) were brought in contact with eluates from freshly mixed and set sealer. The sealers were mixed strictly according to the manufacturers' instructions and identically samples were produced. 1:1, 1:2, and 1:10 dilutions of sealers extract were used. Extracts from freshly mixed sealer were added to the PDL-cells on day one to simulate a clinical scenario. Subsequently, at 24 h, 7, 14, and 21 days extracts form set sealers were used for PDL-cell culturing. PDL-cell viability was analyzed by living-cell-count, MTT-assay, and living/dead-staining, cytotoxicity by LDH-assay, and changes by Richardson-staining. All data were statistically evaluated by one way ANOVA and a posthoc analysis with Bonferroni-Holm testing (p < 0.05). In contact with BioRoot-RCS a regeneration of the PDL-cells were observed over time. This sealer showed the lowest toxicity in a freshly mixed and set state (p < 0.05). MTA-Fillapex and Pulp-Canal-Sealer were cytotoxic in a fresh as well as in a set state, whereas AH-Plus was cytotoxic in a freshly mixed state, but not when the sealer was set. BioRoot-RCS is biocompatible and bioactive because it seems to have a positive influence on the PDL-cell metabolism. Pulp Canal Sealer and MTA-Fillapex showed no biocompatibility in contact with PDL-cells at all. Freshly mixed AH Plus is less biocompatible on PDL than in a set state.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.