Evidence map›Paper›PMID 30038662›Full record

ArticleInfectious agents and cancer2018

Glycogene expression profiles based on microarray data from cervical carcinoma HeLa cells with partially silenced E6 and E7 HPV oncogenes.

Miguel Aco-Tlachi, Ricardo Carreño-López, Patricia L Martínez-Morales, Paola Maycotte, Adriana Aguilar-Lemarroy, Luis Felipe Jave-Suárez, Gerardo Santos-López, Julio Reyes-Leyva, Verónica Vallejo-Ruiz

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infectious agents and cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Miguel Aco-Tlachi1Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km. 4.5 Carretera Federal Atlixco-Metepec, Atlixco, C.P. 74360 Puebla, Mexico.
Ricardo Carreño-López2Posgrado en Ciencias Microbiológicas, Benemérita Universidad Autónoma de Puebla, Edificio 103-J Cd. Universitaria, Col. San Manuel, C.P. 72570 Puebla, Pue Mexico.
Patricia L Martínez-Morales4CONACYT- Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km. 4.5 Carretera Federal Atlixco-Metepec, Atlixco, C.P. 74360 Puebla, Mexico.
Paola Maycotte4CONACYT- Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km. 4.5 Carretera Federal Atlixco-Metepec, Atlixco, C.P. 74360 Puebla, Mexico.
Adriana Aguilar-Lemarroy3Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Col Independencia, C.P. 44340 Guadalajara, Jalisco Mexico.
Luis Felipe Jave-Suárez3Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Col Independencia, C.P. 44340 Guadalajara, Jalisco Mexico.
Gerardo Santos-López1Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km. 4.5 Carretera Federal Atlixco-Metepec, Atlixco, C.P. 74360 Puebla, Mexico.
Julio Reyes-Leyva1Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km. 4.5 Carretera Federal Atlixco-Metepec, Atlixco, C.P. 74360 Puebla, Mexico.
Verónica Vallejo-Ruiz1Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km. 4.5 Carretera Federal Atlixco-Metepec, Atlixco, C.P. 74360 Puebla, Mexico.ORCID 0000-0001-7884-1406
Mexican Social Security Institute · MXBenemérita Universidad Autónoma de Puebla · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAberrant glycosylation is a characteristic of tumour cells. The expression of certain glycan structures has been associated with poor prognosis. In cervical carcinoma, changes in the expression levels of some glycogenes have been associated with lymph invasion. Human papillomavirus (HPV) infection is one of the most important factors underlying the development of cervical cancer. The HPV oncoproteins E6 and E7 have been implicated in cervical carcinogenesis and can modify the host gene expression profile. The roles of these oncoproteins in glycosylation changes have not been previously reported.

methodsTo determine the effect of the E6 and E7 oncoproteins on glycogene expression we partially silenced the E6 and E7 oncogenes in HeLa cells, we performed a microarray expression assay to identify altered glycogenes and quantified the mRNA levels of glycogenes by RT-qPCR. A protein-protein interaction network was constructed to identify potentially altered glycosylation pathways.

resultsThe microarray analysis showed 9 glycogenes that were upregulated and 7 glycogenes that were downregulated in HeLa shE6/E7 cells. Some of these genes participate in glycosylation related to Notch proteins and O-glycans antigens.

conclusionsOur results support that E6 and E7 oncoproteins could modify glycogene expression the products of which participate in the synthesis of structures implicated in proliferation, adhesion and apoptosis.

Indexed as

Cervical cancerE6 oncoproteinE7 oncoproteinGlycogeneHeLa cellsHuman papillomavirusMicroarrays

Identifiers

PMID30038662
PMCPMC6053821
OpenAlexW2884580152

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.