Evidence map›Paper›PMID 30038324›Full record

ArticleBritish journal of cancer2018

Exosome-packaged miR-1246 contributes to bystander DNA damage by targeting LIG4.

Li-Jun Mo, Man Song, Qiao-Hua Huang, Hua Guan, Xiao-Dan Liu, Da-Fei Xie, Bo Huang, Rui-Xue Huang, Ping-Kun Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  20. The emerging role of exosomes in radiotherapy.Cell communication and signaling : CCS · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Li-Jun MoInstitute for Environmental Medicine and Radiation Health, the College of Public Health, University of South China, 421001, Hengyang, Hunan Province, P.R. China.
Man SongBeijing Key Laboratory for Radiobiology, Department of Radiation Biology, Beijing Institute of Radiation Medicine, 100850, Beijing, P.R. China.
Qiao-Hua HuangInstitute for Environmental Medicine and Radiation Health, the College of Public Health, University of South China, 421001, Hengyang, Hunan Province, P.R. China.
Hua GuanBeijing Key Laboratory for Radiobiology, Department of Radiation Biology, Beijing Institute of Radiation Medicine, 100850, Beijing, P.R. China.
Xiao-Dan LiuBeijing Key Laboratory for Radiobiology, Department of Radiation Biology, Beijing Institute of Radiation Medicine, 100850, Beijing, P.R. China.
Da-Fei XieBeijing Key Laboratory for Radiobiology, Department of Radiation Biology, Beijing Institute of Radiation Medicine, 100850, Beijing, P.R. China.
Bo HuangInstitute for Environmental Medicine and Radiation Health, the College of Public Health, University of South China, 421001, Hengyang, Hunan Province, P.R. China.
Rui-Xue HuangDepartment of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, 410078, Changsha, Hunan Province, P.R. China. huangruixue@csu.edu.cn.
Ping-Kun ZhouBeijing Key Laboratory for Radiobiology, Department of Radiation Biology, Beijing Institute of Radiation Medicine, 100850, Beijing, P.R. China. zhoupk@bmi.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAn increasing number of studies have recently reported that microRNAs packaged in exosomes contribute to multiple biological processes such as cancer progression; however, little is known about their role in the development of radiation-induced bystander effects.

methodsThe exosomes were isolated from the culture medium of BEP2D cells with or without γ-ray irradiation by ultracentrifugation. To monitor DNA damage and repair efficiency, the DNA double-strand break biomarker 53BP1 foci, comet, micronuclei, expression of DNA repair genes and NHEJ repair activity were detected. The miR-1246 targeting sequence of the DNA ligase 4 (LIG4) mRNA 3'UTR was assessed by luciferase reporter vectors.

resultsmiR-1246 was increased in exosomes secreted from 2 Gy-irradiated BEP2D cells and inhibited the proliferation of nonirradiated cells. The miR-1246 mimic, exosomes from irradiated cells, and radiation-conditioned cell culture medium increased the yields of 53BP1 foci, comet tail and micronuclei in nonirradiated cells, and decreased NHEJ efficiency. miR-1246 downregulated LIG4 expression by directly targeting its 3'UTR.

conclusionsOur findings demonstrate that miR-1246 packaged in exosomes could act as a transfer messenger and contribute to DNA damage by directly repressing the LIG4 gene. Exosomal miR-1246 may be a critical predictor of and player in radiation-induced bystander DNA damage.

Indexed as

Down-Regulation3' Untranslated RegionsBystander EffectCell LineCell ProliferationCulture Media, ConditionedDNA DamageDNA Ligase ATPExosomesGene Expression RegulationHEK293 CellsHumansMicroRNAsSequence Analysis, DNA3' Untranslated RegionsCulture Media, ConditionedDNA Ligase ATPLIG4 protein, humanMicroRNAsMIRN1246 microRNA, human

Identifiers

PMID30038324
PMCPMC6134031

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.