ArticleScientific reports2018
Fundamental parameters of the developing thymic epithelium in the mouse.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Thymic APC Networks Orchestrate T-Cell Selection: Mechanisms and Therapeutic Opportunities in Immune Disorders.Immunology · 2026Review
- RNA-binding proteins Zfp36l1 and Zfp36l2 protect against premature thymic involution.Cellular & molecular immunology · 2026Article
- Developmental hematopoiesis: innate programming of αβ T cells.Experimental hematology · 2026Review
- Development of αβ and γδ T Cells in the Thymus and Methods of Analysis.International journal of molecular sciences · 2025Review
- Beyond cysts - organization of epithelial networks in the murine thymus.Journal of cell science · 2025Article
- Deconstructing the Thymic Microenvironment Through Genesis to Senescence.Immunological reviews · 2025Review
- Article
- Generation and repair of thymic epithelial cells.The Journal of experimental medicine · 2024Review
- Preparation of Single-cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric Analysis.Journal of visualized experiments : JoVE · 2024Article
- Functionally diverse thymic medullary epithelial cells interplay to direct central tolerance.Cell reports · 2024Article
- Foxn1 overexpression promotes thymic epithelial progenitor cell proliferation and mTEC maintenance, but does not prevent thymic involution.Development (Cambridge, England) · 2023Article
- Type III interferon drives thymic B cell activation and regulatory T cell generation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Large-Scale Isolation of Mouse Thymic Epithelial Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Revelations in Thymic Epithelial Cell Biology and Heterogeneity from Single-Cell RNA Sequencing and Lineage Tracing Methodologies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mesenchymal stromal cells in the thymus.Inflammation and regeneration · 2022Review
- Tissue-specific proteasomes in generation of MHC class I peptides and CD8Current opinion in immunology · 2022Review
- Article
- A 2020 View of Thymus Stromal Cells in T Cell Development.Journal of immunology (Baltimore, Md. : 1950) · 2021Review
- Age-Related Changes in Thymic Central Tolerance.Frontiers in immunology · 2021Review
- High-Oxygen Submersion Fetal Thymus Organ Cultures Enable FOXN1-Dependent and -Independent Support of T Lymphopoiesis.Frontiers in immunology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The numbers of thymic epithelial cells (TECs) and thymocytes steadily increase during embryogenesis. To examine this dynamic, we generated several TEC-specific transgenic mouse lines, which express fluorescent proteins in the nucleus, the cytosol and in the membranes under the control of the Foxn1 promoter. These tools enabled us to determine TEC numbers in tissue sections by confocal fluorescent microscopy, and in the intact organ by light-sheet microscopy. Compared to histological procedures, flow cytometric analysis of thymic cellularity is shown to underestimate the numbers of TECs by one order of magnitude; using enzymatic digestion of thymic tissue, the loss of cortical TECs (cTECs) is several fold greater than that of medullary TECs (mTECs), although different cTEC subsets appear to be still present in the final preparation. Novel reporter lines driven by Psmb11 and Prss16 promoters revealed the trajectory of differentiation of cTEC-like cells, and, owing to the additional facility of conditional cell ablation, allowed us to follow the recovery of such cells after their depletion during embryogenesis. Multiparametric histological analyses indicate that the new transgenic reporter lines not only reveal the unique morphologies of different TEC subsets, but are also conducive to the analysis of the complex cellular interactions in the thymus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.