Evidence map›Paper›PMID 30038254›Full record

ArticleNature cell biology2018

Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures.

Berna Sozen, Gianluca Amadei, Andy Cox, Ran Wang, Ellen Na, Sylwia Czukiewska, Lia Chappell, Thierry Voet, Geert Michel, Naihe Jing and 2 more

Erratum issuedAbstract readComparative StudyVideo-Audio Media
PubMed Publisher
In one paragraph

Article in Nature cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 176 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
176citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

176 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  7. Synthetic embryos: current progress, application and challenges.Reproductive biology and endocrinology : RB&E · 2026
    Review
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116 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Berna Sozen *Mammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0001-5834-5819
Gianluca Amadei *Mammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.
Andy CoxMammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.
Ran WangState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
Ellen NaDepartment of Cardiology, Charité, Berlin, Germany.ORCID http://orcid.org/0000-0003-1395-9707
Sylwia CzukiewskaMammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.
Lia ChappellWellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK.
Thierry VoetWellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK.
Geert MichelTransgene Technologies, Charité, Berlin, Germany.
Naihe JingState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
David M GloverDepartment of Genetics, University of Cambridge, Cambridge, UK.
Magdalena Zernicka-GoetzMammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK. mz205@cam.ac.uk.ORCID http://orcid.org/0000-0002-7004-2471

Funding

Department of Health RP-2014-05-019
6 · The paper itself

Abstract

Embryonic stem cells can be incorporated into the developing embryo and its germ line, but, when cultured alone, their ability to generate embryonic structures is restricted. They can interact with trophoblast stem cells to generate structures that break symmetry and specify mesoderm, but their development is limited as the epithelial-mesenchymal transition of gastrulation cannot occur. Here, we describe a system that allows assembly of mouse embryonic, trophoblast and extra-embryonic endoderm stem cells into structures that acquire the embryo's architecture with all distinct embryonic and extra-embryonic compartments. Strikingly, such embryo-like structures develop to undertake the epithelial-mesenchymal transition, leading to mesoderm and then definitive endoderm specification. Spatial transcriptomic analyses demonstrate that these morphological transformations are underpinned by gene expression patterns characteristic of gastrulating embryos. This demonstrates the remarkable ability of three stem cell types to self-assemble in vitro into gastrulating embryo-like structures undertaking spatio-temporal events of the gastrulating mammalian embryo.

Indexed as

Cell CommunicationEpithelial-Mesenchymal TransitionGastrulationAnimalsCell DifferentiationCell LineCell LineageCell MovementCoculture TechniquesEmbryo, MammalianEndodermGene Expression Regulation, DevelopmentalGestational AgeMiceMouse Embryonic Stem CellsPhenotype

Identifiers

PMID30038254

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.