ArticleScientific reports2018
Falling water ice affinity purification of ice-binding proteins.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Extended Temperature Range of the Ice-Binding Protein Activity.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Ice Shell Purification of Ice-Active Compounds.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Toward Understanding Bacterial Ice Nucleation.The journal of physical chemistry. B · 2022Article
- Structural diversity of marine anti-freezing proteins, properties and potential applications: a review.Bioresources and bioprocessing · 2022Review
- Cold adaptation strategies in plants-An emerging role of epigenetics and antifreeze proteins to engineer cold resilient plants.Frontiers in genetics · 2022Review
- Fish-Derived Antifreeze Proteins and Antifreeze Glycoprotein Exhibit a Different Ice-Binding Property with Increasing Concentration.Biomolecules · 2020Article
- Ice Nucleation Properties of Ice-binding Proteins from Snow Fleas.Biomolecules · 2019Article
- Ice-binding proteins and the applicability and limitations of the kinetic pinning model.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2019Article
- Laboratory-Scale Isolation of Insect Antifreeze Protein for Cryobiology.Biomolecules · 2019Article
- A sudden-melting event during water freezing inside a copper well.RSC advances · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Ice-binding proteins (IBPs) permit their hosts to thrive in the presence of ice. The ability of IBPs to control ice growth makes them potential additives in industries ranging from food storage and cryopreservation to anti-icing systems. For IBPs to be used in commercial applications, however, methods are needed to produce sufficient quantities of high-quality proteins. Here, we describe a new method for IBP purification, termed falling water ice affinity purification (FWIP). The method is based on the affinity of IBPs for ice and does not require molecular tags. A crude IBP solution is allowed to flow over a chilled vertical surface of a commercial ice machine. The temperature of the surface is lowered gradually until ice crystals are produced, to which the IBPs bind but other solutes do not. We found that a maximum of 35 mg of IBP was incorporated in 1 kg of ice. Two rounds of FWIP resulted in >95% purity. An ice machine that produces 60 kg of ice per day can be used to purify one gram of IBP per day. In combination with efficient concentration of the protein solution by tangential flow filtration the FWIP method is suitable for the purification of grams of IBPs for research purposes and applications.
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