ArticleThe Journal of cell biology2018
Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation.
Article in The Journal of cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A systematic interactome ofeLife · 2026Article
- Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2.Nucleic acids research · 2026Article
- Chromosomal R-loops: who R they?Biologia futura · 2024Review
- The COMPASS subunit Spp1 protects nascent DNA at the Tus/Ter replication fork barrier by limiting DNA availability to nucleases.Nature communications · 2023Article
- NODULIN HOMEOBOX is required for heterochromatin homeostasis in Arabidopsis.Nature communications · 2022Article
- H3K4me3 recognition by the COMPASS complex facilitates the restoration of this histone mark following DNA replication.Science advances · 2022Article
- Chromosome architecture and homologous recombination in meiosis.Frontiers in cell and developmental biology · 2022Review
- Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation.eLife · 2021Article
- Sharing Marks: H3K4 Methylation and H2B Ubiquitination as Features of Meiotic Recombination and Transcription.International journal of molecular sciences · 2020Review
- Genetic Interactions of Histone Modification Machinery Set1 and PAF1C with the Recombination Complex Rec114-Mer2-Mei4 in the Formation of Meiotic DNA Double-Strand Breaks.International journal of molecular sciences · 2020Article
- Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans.Nucleic acids research · 2019Article
- Histone H3 Lysine 56 Acetylation Is Required for Formation of Normal Levels of Meiotic DNA Breaks inFrontiers in cell and developmental biology · 2019Article
- Commentary: Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation.Frontiers in genetics · 2018Article
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11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spp1 is the H3K4me3 reader subunit of the Set1 complex (COMPASS/Set1C) that contributes to the mechanism by which meiotic DNA break sites are mechanistically selected. We previously proposed a model in which Spp1 interacts with H3K4me3 and the chromosome axis protein Mer2 that leads to DSB formation. Here we show that spatial interactions of Spp1 and Mer2 occur independently of Set1C. Spp1 exhibits dynamic chromatin binding features during meiosis, with many de novo appearing and disappearing binding sites. Spp1 chromatin binding dynamics depends on its PHD finger and Mer2-interacting domain and on modifiable histone residues (H3R2/K4). Remarkably, association of Spp1 with Mer2 axial sites reduces the effective turnover rate and diffusion coefficient of Spp1 upon chromatin binding, compared with other Set1C subunits. Our results indicate that "chromosomal turnover rate" is a major molecular determinant of Spp1 function in the framework of meiotic chromatin structure that prepares recombination initiation sites for break formation.
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