Evidence map›Paper›PMID 30036883›Full record

ArticleCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology2018

Regulatory Role of Endothelial PHD2 in the Hepatic Steatosis.

Li-Ying Zhou, Heng Zeng, Shuo Wang, Jian-Xiong Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Role of liver sinusoidal endothelial cells in liver diseases.Nature reviews. Gastroenterology & hepatology · 2021
    Review
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Li-Ying ZhouDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, School of Medicine, Jackson, Mississippi, USA.
Heng ZengDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, School of Medicine, Jackson, Mississippi, USA.
Shuo WangDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, School of Medicine, Jackson, Mississippi, USA.
Jian-Xiong ChenDepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, School of Medicine, Jackson, Mississippi, USA.
University of Mississippi Medical Center · USBeijing Chao-Yang Hospital · CNBeijing Obstetrics and Gynecology Hospital · CN

Funding

Regulation of vascular maturation/regression in diabetesR01HL102042 · NHLBI · VANDERBILT UNIVERSITY · PI CHEN, JIAN-XIONG · 2010 to 2020
$3.0M
NHLBI NIH HHS R01 HL102042
6 · The paper itself

Abstract

BACKGROUND/

aimsLiver disease is a leading cause of high mortality and morbidity worldwide. The aim of the present study is to investigate the regulatory role of prolyl hydroxylase-2 (PHD2)-hypoxia-inducible factor-2a (HIF-2α) axis on nonalcoholic fatty liver disease (NAFLD) and to explore the potential mechanisms by which endothelial (EC)-specific PHD2 deficiency regulates hepatic steatosis and fibrosis.

methodsIn the endothelial-specific PHD2 knockout (PHD2ECKO) mouse fed with normal diet or high fat diet (HFD), liver lipid accumulation and fibrosis were measured by Oil Red O and Masson trichrome staining. The fat and body weight (FW/BW) ratio and glucose tolerance were measured. The expression of HIF-2α, atrial natriuretic peptide (ANP), angiopoietin-2 (Ang-2), and transforming growth factor-b (TGF-β) were analyzed by western blot analysis.

resultsThe steatosis and fibrosis were significantly increased in the PHD2ECKO mice. FW/BW ratio was significantly increased in the PHD2ECKO mice. Moreover, knockout of endothelial PHD2 resulted in an impairment of glucose tolerance in mice. Western blot analysis showed that the expression of HIF-2α in liver tissues was not significantly increased. Interestingly, the expression of ANP was decreased, and Ang-2 and TGF-β levels were significantly increased in the liver of PHD2ECKO mice. The FW/BW ratio was also significantly increased in the PHD2ECKO mice fed with HFD for 16 weeks. Feeding HFD resulted in a significant increase in hepatic steatosis in the control PHD2f/f mice, but did not further enhance hepatic steatosis in the PHD2ECKO mice.

conclusionsWe concluded that the endothelial PHD2 plays a critical role in hepatic steatosis and fibrosis, which may be involved in the regulation of ANP and Ang-2/TGF-β signaling pathway, but not the HIF-2α expression.

Indexed as

AnimalsAtrial Natriuretic FactorBasic Helix-Loop-Helix ProteinsDiet, High-FatEndothelial PAS Domain-Containing Protein 1EndotheliumGlucose Tolerance TestHypoxia-Inducible Factor-Proline DioxygenasesLiverMiceMice, KnockoutNon-alcoholic Fatty Liver DiseasePhosphofructokinase-2Proto-Oncogene Proteins c-aktRibonuclease, PancreaticSignal TransductionAng2 protein, mouseAtrial Natriuretic FactorBasic Helix-Loop-Helix ProteinsEgln1 protein, mouseEndothelial PAS Domain-Containing Protein 1Hypoxia-Inducible Factor-Proline DioxygenasesPFKFB3 protein, mousePhosphofructokinase-2Proto-Oncogene Proteins c-aktRibonuclease, PancreaticTransforming Growth Factor betaAtrial natriuretic peptide (ANP)EndotheliumHepatic SteatosisHIF-2αPhd2

Identifiers

PMID30036883
PMCPMC6350253
OpenAlexW2884951476

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.