ArticleJournal of controlled release : official journal of the Controlled Release Society2018
Quantitative imaging of receptor-ligand engagement in intact live animals.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
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- Deep learning-based temporal deconvolution for photon time-of-flight distribution retrieval.Optics letters · 2024Article
- Fluorescence Lifetime Imaging for Quantification of Targeted Drug Delivery in Varying Tumor Microenvironments.bioRxiv : the preprint server for biology · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
- In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET.Biophysical reports · 2023Article
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- Monte Carlo-based data generation for efficient deep learning reconstruction of macroscopic diffuse optical tomography and topography applications.Journal of biomedical optics · 2022Article
- Deep learning in macroscopic diffuse optical imaging.Journal of biomedical optics · 2022Review
- Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug-Target Engagement.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Deep Learning in Biomedical Optics.Lasers in surgery and medicine · 2021Review
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- Modeling Pharmacokinetics and Pharmacodynamics of Therapeutic Antibodies: Progress, Challenges, and Future Directions.Pharmaceutics · 2021Review
- FLIM data analysis based on Laguerre polynomial decomposition and machine-learning.Journal of biomedical optics · 2021Article
- Quantification of Trastuzumab-HER2 Engagement In Vitro and In Vivo.Molecules (Basel, Switzerland) · 2020Article
- High compression deep learning based single-pixel hyperspectral macroscopic fluorescence lifetime imagingBiomedical optics express · 2020Article
- Quantitative label-free imaging of iron-bound transferrin in breast cancer cells and tumors.Redox biology · 2020Article
- UNMIX-ME: spectral and lifetime fluorescence unmixing via deep learning.Biomedical optics express · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Maintaining an intact tumor environment is critical for quantitation of receptor-ligand engagement in a targeted drug development pipeline. However, measuring receptor-ligand engagement in vivo and non-invasively in preclinical settings is extremely challenging. We found that quantitation of intracellular receptor-ligand binding can be achieved using whole-body macroscopic lifetime-based Förster Resonance Energy Transfer (FRET) imaging in intact, live animals bearing tumor xenografts. We determined that FRET levels report on ligand binding to transferrin receptors conversely to raw fluorescence intensity. FRET levels in heterogeneous tumors correlate with intracellular ligand binding but strikingly, not with ubiquitously used ex vivo receptor expression assessment. Hence, MFLI-FRET provides a direct measurement of systemic delivery, target availability and intracellular drug delivery in preclinical studies. Here, we have used MFLI to measure FRET longitudinally in intact and live animals. MFLI-FRET is well-suited for guiding the development of targeted drug therapy in heterogeneous tumors in intact, live small animals.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.