Evidence map›Paper›PMID 30035770›Full record

ArticleJournal of visualized experiments : JoVE2018

Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms.

Skyler Hunter, Malabika Maulik, Courtney Scerbak, Elena Vayndorf, Barbara E Taylor

Open access · bronzeAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Microfluidic-AssistedCyborg and bionic systems (Washington, D.C.) · 2023
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Skyler HunterDepartment of Biology and Wildlife, University of Alaska Fairbanks; Institute of Arctic Biology, University of Alaska Fairbanks.
Malabika MaulikDepartment of Chemistry and Biochemistry, University of Alaska Fairbanks; mmaulik@alaska.edu.
Courtney ScerbakDepartments of Biology and Chemistry, Earlham College.
Elena VayndorfInstitute of Arctic Biology, University of Alaska Fairbanks.
Barbara E TaylorDepartment of Biological Sciences, College of Natural Science and Mathematics, California State University Long Beach.
University of Alaska Fairbanks · USCalifornia State University, Long Beach · USEarlham College · US

Funding

The Administrative CoreP20GM103395 · NIGMS · UNIVERSITY OF ALASKA FAIRBANKS · PI BRIAN M BARNES · 2012 to 2026
$66.1M
Biomedical Learning and Student Training (BLaST) ProgramUL1GM118991 · NIGMS · UNIVERSITY OF ALASKA FAIRBANKS · PI HUEFFER, KARSTEN · 2015 to 2023
$19.0M
Biomedical Learning and Student Training (BLaST) ProgramRL5GM118990 · NIGMS · UNIVERSITY OF ALASKA FAIRBANKS · PI HUEFFER, KARSTEN, REYNOLDS, ARLEIGH JAMES · 2015 to 2023
$10.5M
Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Ann E. Rougvie · 2012 to 2026
$7.5M
Biomedical Learning and Student Training (BLaST) ProgramTL4GM118992 · NIGMS · UNIVERSITY OF ALASKA FAIRBANKS · PI HUEFFER, KARSTEN, REYNOLDS, ARLEIGH JAMES · 2015 to 2023
$7.1M
NIGMS NIH HHS P20 GM103395NIGMS NIH HHS RL5 GM118990NIGMS NIH HHS TL4 GM118992NIGMS NIH HHS UL1 GM118991NIH HHS P40 OD010440
6 · The paper itself

Abstract

Caenorhabditis elegans (C. elegans) is a well-established model organism used across a range of basic and biomedical research. Within the nematode research community, there is a need for an affordable and effective way to maintain large, age-matched populations of C. elegans. Here, we present a methodology for mechanically sorting and cleaning C. elegans. Our aim is to provide a cost-effective, efficient, fast, and simple process to obtain animals of uniform sizes and life stages for their use in experiments. This tool, the Caenorhabditis Sieve, uses a custom-built lid system that threads onto common conical lab tubes and sorts C. elegans based on body size. We also demonstrate that the Caenorhabditis Sieve effectively transfers animals from one culture plate to another allowing for a rapid sorting, synchronizing, and cleaning without impacting markers of health, including motility and stress-inducible gene reporters. This accessible and innovative tool is a fast, efficient, and non-stressful option for maintaining C. elegans populations.

Indexed as

AnimalsCaenorhabditis

Identifiers

PMID30035770
PMCPMC6124601
OpenAlexW2872103253

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.