ArticleAngewandte Chemie (International ed. in English)2018
The Broad Aryl Acid Specificity of the Amide Bond Synthetase McbA Suggests Potential for the Biocatalytic Synthesis of Amides.
Article in Angewandte Chemie (International ed. in English), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Next-Generation Manufacturing: The Evolving Role of Biocatalysis in AstraZeneca.Chembiochem : a European journal of chemical biology · 2026Review
- Biocatalysis Under Reduced Pressure; Two-Step, One-Pot Amide Synthesis Using an Immobilised Transaminase/Lipase Cascade in Combination With By-Product Removal.Chembiochem : a European journal of chemical biology · 2026Article
- Enzymatic aminolysis in continuous flow: an efficient strategy for the synthesis of salicylamide derivatives.RSC advances · 2026Article
- A tailored CoA ligase-Nature catalysis · 2026Article
- Enzymatic combinatorial synthesis of E-64 and related cysteine protease inhibitors.Nature chemical biology · 2025Article
- Genome Mining of Isoindolinone-Containing Peptide Natural Products.Journal of the American Chemical Society · 2025Article
- Enzymatic Cascades for Stereoselective and Regioselective Amide Bond Assembly.Angewandte Chemie (International ed. in English) · 2025Article
- Accelerated enzyme engineering by machine-learning guided cell-free expression.Nature communications · 2025Article
- Development of the Esterase PestE for Amide Bond Synthesis Under Aqueous Conditions: Enzyme Cascades for Converting Waste PET into Tamibarotene.Angewandte Chemie (International ed. in English) · 2025Article
- Pyrazine derivative synthesis in a continuous-flow system: a green synthesis of pyrazinamide from pyrazine esters and amines catalyzed by Lipozyme® TL IM fromRSC advances · 2024Article
- NewSynthetic and systems biotechnology · 2024Article
- Cryptic enzymatic assembly of peptides armed with β-lactone warheads.Nature chemical biology · 2024Article
- Broad Spectrum Enantioselective Amide Bond Synthetase fromACS catalysis · 2024Article
- Recent Developments and Applications of Biocatalytic and Chemoenzymatic Synthesis for the Generation of Diverse Classes of Drugs.Current pharmaceutical biotechnology · 2024Article
- Semirational engineering ofApplied and environmental microbiology · 2023Article
- Direct formation of amide/peptide bonds from carboxylic acids: no traditional coupling reagents, 1-pot, and green.Chemical science · 2023Article
- One-Step Biocatalytic Synthesis of Sustainable Surfactants by Selective Amide Bond Formation.Angewandte Chemie (International ed. in English) · 2022Article
- Recent Advances in Biocatalysis for Drug Synthesis.Biomedicines · 2022Review
- The Transformative Power of Biocatalysis in Convergent Synthesis.Journal of the American Chemical Society · 2022Review
- Enzymkatalysierte späte Modifizierungen: Besser spät als nie.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Review
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Abstract
Amide bond formation is one of the most important reactions in pharmaceutical synthetic chemistry. The development of sustainable methods for amide bond formation, including those that are catalyzed by enzymes, is therefore of significant interest. The ATP-dependent amide bond synthetase (ABS) enzyme McbA, from Marinactinospora thermotolerans, catalyzes the formation of amides as part of the biosynthetic pathway towards the marinacarboline secondary metabolites. The reaction proceeds via an adenylate intermediate, with both adenylation and amidation steps catalyzed within one active site. In this study, McbA was applied to the synthesis of pharmaceutical-type amides from a range of aryl carboxylic acids with partner amines provided at 1-5 molar equivalents. The structure of McbA revealed the structural determinants of aryl acid substrate tolerance and differences in conformation associated with the two half reactions catalyzed. The catalytic performance of McbA, coupled with the structure, suggest that this and other ABS enzymes may be engineered for applications in the sustainable synthesis of pharmaceutically relevant (chiral) amides.
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