ArticleProteomics2018
Assessment and Refinement of Sample Preparation Methods for Deep and Quantitative Plant Proteome Profiling.
Article in Proteomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Toward simple, rapid, and deep plant proteome analysis with an in-cell proteomics strategy.Plant physiology · 2026Article
- Multi-proteomic profiling indicates potential regulatory signatures underlying rice resistance to Magnaporthe oryzae.The Plant journal : for cell and molecular biology · 2026Article
- AI-integrated digital breeding for crop improvement.Frontiers in plant science · 2026Review
- Dynamic ASK1 proximity networks uncover SCF-dependent and noncanonical roles in ABA and drought adaptation.bioRxiv : the preprint server for biology · 2025Article
- GC3 codons enhance protein production in diverse GC- and AT-rich plant species.bioRxiv : the preprint server for biology · 2025Article
- Toward simple, rapid, and deep plant proteome analysis with an in-cell proteomics strategy.bioRxiv : the preprint server for biology · 2025Article
- Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.The Plant cell · 2024Article
- Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry.ACS measurement science au · 2024Review
- Genome-wide analysis of mRNA decay in Arabidopsis shoot and root reveals the importance of co-translational mRNA decay in the general mRNA turnover.Nucleic acids research · 2024Article
- ZmPILS6 is an auxin efflux carrier required for maize root morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Improved super-resolution ribosome profiling reveals prevalent translation of upstream ORFs and small ORFs in Arabidopsis.The Plant cell · 2024Article
- Getting Ready for Large-Scale Proteomics in Crop Plants.Nutrients · 2023Article
- Detergent-Assisted Protein Digestion-On the Way to Avoid the Key Bottleneck of Shotgun Bottom-Up Proteomics.International journal of molecular sciences · 2022Review
- Integration of multi-omics data reveals interplay between brassinosteroid and Target of Rapamycin Complex signaling in Arabidopsis.The New phytologist · 2022Article
- Integrated omics reveal novel functions and underlying mechanisms of the receptor kinase FERONIA in Arabidopsis thaliana.The Plant cell · 2022Article
- Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant-Pathogen Interactions.Proteomes · 2022Review
- Integrated omics networks reveal the temporal signaling events of brassinosteroid response in Arabidopsis.Nature communications · 2021Article
- Mapping the plant proteome: tools for surveying coordinating pathways.Emerging topics in life sciences · 2021Review
- SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS.Frontiers in plant science · 2021Article
- Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses.The Plant cell · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A major challenge in the field of proteomics is obtaining high-quality peptides for comprehensive proteome profiling by LC-MS. Here, evaluation and modification of a range of sample preparation methods using photosynthetically active Arabidopsis leaf tissue are done. It was found that inclusion of filter-aided sample preparation (FASP) based on filter digestion improves all protein extraction methods tested. Ultimately, a detergent-free urea-FASP approach that enables deep and robust quantification of leaf and root proteomes is shown. For example, from 4-day-old leaf tissue, up to 11 690 proteins were profiled from a single sample replicate. This method should be broadly applicable to researchers working with difficult to process plant samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.