Evidence map›Paper›PMID 30032837›Full record

ArticleLung cancer (Amsterdam, Netherlands)2018

Epithelial-to-mesenchymal transition of A549 lung cancer cells exposed to electronic cigarettes.

Atena Zahedi, Rattapol Phandthong, Angela Chaili, Guadalupe Remark, Prue Talbot

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lung cancer (Amsterdam, Netherlands), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed, 2 pooled it
2.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 2 syntheses or guidelines pooled it, 55 citations in OpenAlex.

  1. Pooled it
  2. Pulmonary effects of e-liquid flavors: a systematic review.Journal of toxicology and environmental health. Part B, Critical reviews · 2022
    Pooled it
  3. Article
  4. Toxicity and Appeal of Flavoured E-Cigarettes and Flavour Ban Outcomes: A Narrative Review.International journal of environmental research and public health · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Atena ZahediBioengineering Graduate Program, University of California, Riverside, California, USA; Department of Molecular, Cell and Systems Biology, University of California, Riverside, California, USA.
Rattapol PhandthongDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, California, USA.
Angela ChailiDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, California, USA.
Guadalupe RemarkDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, California, USA.
Prue TalbotBioengineering Graduate Program, University of California, Riverside, California, USA; Department of Molecular, Cell and Systems Biology, University of California, Riverside, California, USA. Electronic address: talbot@ucr.edu.
University of California, Riverside · US

Funding

Metals in Electronic Cigarette Aerosol R01DA036493 · NIDA · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI TALBOT, PRUDENCE · 2014 to 2018
$3.6M
Do Electronic Cigarettes Reduce Harm?R21DA037365 · NIDA · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI TALBOT, PRUDENCE · 2014 to 2015
$408k
NIDA NIH HHS R01 DA036493NIDA NIH HHS R21 DA037365
6 · The paper itself

Abstract

objectivesEpithelial-to-mesenchymal transition (EMT) is the initial step enabling the metastasis of cancer cells, which often leads to death. Although smoking is a major risk factor for lung cancer, there is still widespread use of conventional cigarettes. Recently, the tobacco industry has been transformed by the introduction of electronic cigarettes (ECs), which have lower levels of carcinogens and may provide a safer alternative. Here, we investigate the ability of EC liquids and aerosols to induce an EMT in A549 lung cancer cells. MATERIALS AND

methodsHuman adenocarcinoma alveolar basal epithelial cells (A549) were exposed to EC liquids and aerosols from a popular product for 3-8 days. Live cell imaging, EMT biomarker analysis, and machine learning/image processing algorithms were used to characterize changes associated with EMT.

resultsLong-term exposure of A549 cells to menthol or tobacco-flavored EC liquids or aerosols induced an EMT that was characterized by acquisition of a fibroblast-like morphology, loss of cell-to-cell junctions, internalization of E-cadherin, increased motility, and upregulation of other EMT markers. The EMT was concurrent with plasma membrane to nuclear translocation of active β-catenin.

conclusionThis is the first known study to show an EMT of lung cancer cells during exposure to EC products. Because an EMT is an initial step leading to metastasis, an intractable problem that often leads to patient death, this critical finding has significant implications for former or heavy cigarette smokers who are using EC and may be at risk for lung cancer or who may already have a lung tumor.

Indexed as

Electronic Nicotine Delivery SystemsA549 CellsAdenocarcinomaCell MovementCell ProliferationEpithelial CellsEpithelial-Mesenchymal TransitionFibroblastsGene Expression Regulation, NeoplasticHumansLung NeoplasmsNeoplasm MetastasisElectronic cigaretteEpithelial-to-mesenchymal transitionLung cancerMetastasisTobacco productsβ-catenin

Identifiers

PMID30032837
PMCPMC6397801
OpenAlexW2808349922

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.