ArticleMolecular biology reports2018
Using response surface methodology in combination with Plackett-Burman design for optimization of culture media and extracellular expression of Trichoderma reesei synthetic endoglucanase II in Escherichia coli.
Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Overproduction of D-pantothenic acid via fermentation conditions optimization and isoleucine feeding from recombinant3 Biotech · 2021Article
- Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis ofPharmaceuticals (Basel, Switzerland) · 2021Article
- Intra-Laboratory Validation of Alpha-Galactosidase Activity Measurement in Dietary Supplements.Molecules (Basel, Switzerland) · 2021Article
- Purification and Enzymatic Properties of a Difunctional Glycoside Hydrolase fromIndian journal of microbiology · 2020Article
- Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology.AMB Express · 2019Article
- Evaluation of Immunogenicity, Protective Immunity on Aquaculture PathogenicIranian journal of biotechnology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellulases like endoglucanase II (EGII) from Trichoderma reesei are the industrial enzymes responsible for breakdown of cellulosic materials. Due to its importance for production of eco-friendly commercial products such as alternative biofuels, industrial EGII production and optimization of its production conditions merit consideration. The gene responsible for EGII expression was designed and sub-cloned in to pET26b expression vector and transformed into BL21 (DE3) pLysS cells. Protein expression and purification was followed by a RSM design (20 experiments) to optimize the IPTG Concentration, post induction period and cell density (OD
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.