Evidence map›Paper›PMID 30030774›Full record

ArticleMolecular biology reports2018

HIRA directly targets the enhancers of selected cardiac transcription factors during in vitro differentiation of mouse embryonic stem cells.

Rasha Noureldin M Saleh, Daniel Dilg, Abla A Abou Zeid, Doaa I Hashad, Peter J Scambler, Ariane L A Chapgier

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Cell Fate Decisions in the Wake of Histone H3 Deposition.Frontiers in cell and developmental biology · 2021
    Review
  6. Homeobox gene Meis1 modulates cardiovascular regeneration.Seminars in cell & developmental biology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Rasha Noureldin M SalehDevelopmental Biology of Birth Defects Section, Institute of Child Health, University College London, London, UK.
Daniel DilgDevelopmental Biology of Birth Defects Section, Institute of Child Health, University College London, London, UK.
Abla A Abou ZeidClinical Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Doaa I HashadClinical Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Peter J ScamblerDevelopmental Biology of Birth Defects Section, Institute of Child Health, University College London, London, UK. p.scambler@ucl.ac.uk.
Ariane L A ChapgierDevelopmental Biology of Birth Defects Section, Institute of Child Health, University College London, London, UK. a.chapgier@ucl.ac.uk.ORCID http://orcid.org/0000-0002-1734-0744
Alexandria University · EGInstitute of Child Health · INUniversity College London · GB

Funding

British Heart Foundation FS/10/031/28395British Heart Foundation RG/15/14/31880Fondation Leducq TNE 15CVD01Great Ormond Street Hospital Charity V2318
6 · The paper itself

Abstract

HIRA is a histone chaperone known to modulate gene expression through the deposition of H3.3. Conditional knockout of Hira in embryonic mouse hearts leads to cardiac septal defects. Loss of function mutation in HIRA, together with other chromatin modifiers, was found in patients with congenital heart diseases. However, the effects of HIRA on gene expression at earlier stages of cardiogenic mesoderm differentiation have not yet been studied. Differentiation of mouse embryonic stem cells (mESCs) towards cardiomyocytes mimics some of these early events and is an accepted model of these early stages. We performed RNA-Seq and H3.3-HA ChIP-seq on both WT and Hira-null mESCs and early cardiomyocyte progenitors of both genotypes. Analysis of RNA-seq data showed differential down regulation of cardiovascular development-related genes in Hira-null cardiomyocytes compared to WT cardiomyocytes. We found HIRA-dependent H3.3 deposition at these genes. In particular, we observed that HIRA influenced directly the expression of the transcription factors Gata6, Meis1 and Tbx2, essential for cardiac septation, through H3.3 deposition. We therefore identified new direct targets of HIRA during cardiac differentiation.

Indexed as

AnimalsCell Cycle ProteinsCell DifferentiationCell LineDown-RegulationEnhancer Elements, GeneticGATA6 Transcription FactorHeart Septal DefectsHistone ChaperonesHistonesLoss of Function MutationMiceMouse Embryonic Stem CellsMyeloid Ecotropic Viral Integration Site 1 ProteinMyocytes, CardiacSequence Analysis, RNACell Cycle ProteinsGata6 protein, mouseGATA6 Transcription FactorHira protein, mouseHistone ChaperonesHistonesMeis1 protein, mouseMyeloid Ecotropic Viral Integration Site 1 ProteinT-Box Domain Protein 2T-Box Domain ProteinsTranscription FactorsCardiomyocytesDifferentiationEmbryonic stem cellsEnhancersH3.3HeartHIRA

Identifiers

PMID30030774
PMCPMC6156767
OpenAlexW2884758747

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.