Evidence map›Paper›PMID 30030768›Full record

ArticleIn vitro cellular & developmental biology. Animal2018

Directed differentiation of mouse P19 embryonal carcinoma cells to neural cells in a serum- and retinoic acid-free culture medium.

Isha Verma, Polani B Seshagiri

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Isha VermaDepartment of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Sir CV Raman Road, Bangalore, 560012, India.
Polani B SeshagiriDepartment of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Sir CV Raman Road, Bangalore, 560012, India. polani@iisc.ac.in.

Funding

Department of Biotechnology , Ministry of Science and Technology BT/PR5425/AAQ/1/498/2012
6 · The paper itself

Abstract

P19 embryonal carcinoma cells (EC-cells) provide a simple and robust culture system for studying neural development. Most protocols developed so far for directing neural differentiation of P19 cells depend on the use of culture medium supplemented with retinoic acid (RA) and serum, which has an undefined composition. Hence, such protocols are not suitable for many molecular studies. In this study, we achieved neural differentiation of P19 cells in a serum- and RA-free culture medium by employing the knockout serum replacement (KSR) supplement. In the KSR-containing medium, P19 cells underwent predominant differentiation into neural lineage and by day 12 of culture, neural cells were present in 100% of P19-derived embryoid bodies (EBs). This was consistently accompanied by the increased expression of various neural lineage-associated markers during the course of differentiation. P19-derived neural cells comprised of NES

Indexed as

Cell DifferentiationAnimalsBiomarkersCulture Media, Serum-FreeEmbryonal Carcinoma Stem CellsEndodermGene Expression RegulationMesodermMiceNeuronsTretinoinBiomarkersCulture Media, Serum-FreeTretinoinDifferentiationEmbryoid bodyEmbryonal carcinoma cellsKnockout serum replacementNeural cellsP19 cells

Identifiers

PMID30030768

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.