Evidence map›Paper›PMID 30030234›Full record

ArticleApplied and environmental microbiology2018

Functional Genetic Elements for Controlling Gene Expression in Cupriavidus necator H16.

Swathi Alagesan, Erik K R Hanko, Naglis Malys, Muhammad Ehsaan, Klaus Winzer, Nigel P Minton

Open access · hybridAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 60 citations in OpenAlex.

  1. Synthetic and systems biotechnology · 2026
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  9. A promoter library for tuning gene expression inFrontiers in bioengineering and biotechnology · 2025
    Article
  10. Review
  11. Synthetic microbiology in sustainability applications.Nature reviews. Microbiology · 2024
    Review
  12. N-terminal truncation of PhaCMicrobial cell factories · 2024
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  14. Review
  15. COMicrobial cell factories · 2024
    Article
  16. Article
  17. Article
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  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Swathi Alagesan *BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United Kingdom.
Erik K R Hanko *BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United Kingdom.
Naglis Malys *BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United Kingdom naglis.malys@nottingham.ac.uk nigel.minton@nottingham.ac.uk.
Muhammad EhsaanBBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United Kingdom.
Klaus WinzerBBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United Kingdom.
Nigel P MintonBBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United Kingdom naglis.malys@nottingham.ac.uk nigel.minton@nottingham.ac.uk.
Biotechnology and Biological Sciences Research Council · GBEngineering and Physical Sciences Research Council · GBUniversity of Nottingham · GB

Funding

Biotechnology and Biological Sciences Research Council BB/L013940/1
6 · The paper itself

Abstract

A robust and predictable control of gene expression plays an important role in synthetic biology and biotechnology applications. Development and quantitative evaluation of functional genetic elements, such as constitutive and inducible promoters as well as ribosome binding sites (RBSs), are required. In this study, we designed, built, and tested promoters and RBSs for controlling gene expression in the model lithoautotroph

Indexed as

Gene Expression Regulation, BacterialRegulatory Sequences, Nucleic AcidBacterial ProteinsBase SequenceCupriavidus necatorDNA, BacterialNucleic Acid ConformationPromoter Regions, GeneticRhamnoseBacterial ProteinsDNA, BacterialRhamnoseCupriavidus necator H16functional genetic elementgene expressionisoprenepromoterribosome binding site

Identifiers

PMID30030234
PMCPMC6146998
OpenAlexW2884086582

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.