ArticleApplied and environmental microbiology2018
Isolation of Previously Uncultured Slow-Growing Bacteria by Using a Simple Modification in the Preparation of Agar Media.
Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 93 citations in OpenAlex.
- Isolation of Rhizospheric Bacteroidota Using Oligotrophic Supplemented Culture Media.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Leaf mold extract agar facilitates recovery of soil bacterial diversity beyond Bacillus dominance.Applied microbiology and biotechnology · 2026Article
- Article
- Sulfur Oxidation by New and Non-Canonical Bacteria in a Subsurface Flow Constructed Wetland Treating Domestic Wastewater.Microorganisms · 2026Article
- Variations in deep-sea microbial composition and assembly mechanisms under different culture strategies.Frontiers in microbiology · 2026Article
- Overcoming gelling agent-derived inhibitory factors enables the cultivation of fastidious nitrifiers on solid media.ISME communications · 2026Article
- An enhanced domestication method for uncultured bacteria.ISME communications · 2026Article
- Mining Microbial Dark Matter: Advanced Cultivation Techniques for Bioactive Compound Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Review
- A diffusion-based integrative approach for culturing previously uncultured bacteria from marine sediments.Marine life science & technology · 2025Article
- Last Decade Insights in Exploiting Marine Microorganisms as Sources of New Bioactive Natural Products.Marine drugs · 2025Review
- Article
- NovelFrontiers in microbiology · 2025Article
- Human microbiota-associated animal models: a review.Frontiers in cellular and infection microbiology · 2025Review
- The familyFrontiers in microbiology · 2025Review
- Article
- Harnessing iChip technology for novel antibiotic discovery from peat soil microbiomes to combat antimicrobial resistance.Frontiers in microbiology · 2025Article
- Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota.Nature communications · 2024Article
- Complete genome sequence ofMicrobiology resource announcements · 2024Article
- Amplicon-guided isolation and cultivation of previously uncultured microbial species from activated sludge.Applied and environmental microbiology · 2023Article
- Enhancing the Conventional Culture: the Evaluation of Several Culture Media and Growth Conditions Improves the Isolation of Ruminal Bacteria.Microbial ecology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most microorganisms living in the environment have yet to be cultured, owing at least in part to their slow and poor propagation properties and susceptibility to oxidative stress. Our previous studies demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving the phosphate and agar separately (termed "PS" medium), can greatly improve the culturability of microorganisms by mitigating oxidative stress compared with the use of "PT" medium (autoclaving the phosphate and agar together). Here, we attempted to isolate phylogenetically novel bacteria by combining PS medium with prolonged cultivation. After inoculation with forest soil or pond sediment samples, significantly more colonies appeared on PS medium than on PT medium. A total of 98 and 74 colonies that emerged after more than 7 days of cultivation were isolated as slow growers from PS and PT media, respectively. Sequencing analysis of their 16S rRNA genes revealed that the slow growers recovered from PS medium included more phylogenetically novel bacteria than those from PT medium, including a strain that could be classified into a novel order in the class
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.