Evidence map›Paper›PMID 30029659›Full record

ArticleJournal of translational medicine2018

Novel distance-progesterone-combined selection approach improves human sperm quality.

Kun Li, Rui Li, Ya Ni, Peibei Sun, Ye Liu, Dan Zhang, Hefeng Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
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  10. Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes.Frontiers in cell and developmental biology · 2019
    Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kun LiDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.
Rui LiDepartment of Reproductive Physiology, Zhejiang Academy of Medical Sciences, Hangzhou, 310013, China.
Ya NiDepartment of Reproductive Physiology, Zhejiang Academy of Medical Sciences, Hangzhou, 310013, China.
Peibei SunDepartment of Reproductive Physiology, Zhejiang Academy of Medical Sciences, Hangzhou, 310013, China.
Ye LiuInternational Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.
Dan ZhangDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.
Hefeng HuangDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China. hhf57@zju.edu.cn.ORCID 0000-0002-0195-985X

Funding

the National Key Research and Development Program of China 2017YFC1001301the Natural Science Foundation of China 31471405the Natural Science Foundation of China 81000244the Natural Science Foundation of China 81490742the Natural Science Foundation of China 81661128010the Natural Science Foundation of China 81771647the Natural Science Foundation of Zhejiang Province LY14H040012the Science and Technology Department of Zhejiang Province 2013C31066the Science and Technology Department of Zhejiang Province 2018C37126
6 · The paper itself

Abstract

backgroundSperm selection is essential for the health of offspring conceived via assistive reproductive technology (ART). Various methods of sperm preparation for in vitro fertilization and intracytoplasmic sperm injection have been developed to acquire sperm with better quality and to avoid potential genetic disorders. However, current sperm processing and selection techniques bypass the natural selection that occurs during fertilization in vivo. The aim of this study was to present a novel distance-progesterone-combined selection approach with an original device based on the human female reproductive tract, and to report on its effectiveness based on sperm progressive motility, as well as chemotaxis.

methodsA novel device with long distance channels which mimicked the female human reproductive system was designed and fabricated. This ready-to-be-used device was developed using a progesterone gradient and human tube fluid media. Sperm swam for 150 min in the device under conditions of 37 °C air temperature with 5% CO

resultsOur distance-progesterone-combined sperm selection method was successfully established. After sperm were selected, the percentage of sperm with normal morphology increased (before vs. after selection, 11.2 ± 1.3% vs. 40.3 ± 6.6%, P = 0.000), the percentage of sperm with DNA fragmentation decreased (before vs. after selection, 15.4 ± 4.0% vs. 6.8 ± 3.3%, P = 0.001), and the percentage of sperm with apoptosis did not change significantly.

conclusionsOur newly-developed method is capable of successfully selecting sperm of high quality. The method will be benefit clinical ART practice as it can reduce sperm-related genetic risks.

Indexed as

ApoptosisChemotaxisDNA FragmentationHumansMaleProgesteroneSpermatozoaProgesteroneApoptosisARTChemotaxisDNA fragmentationMorphologyProgesteroneSpermSperm motilitySperm qualitySperm selection

Identifiers

PMID30029659
PMCPMC6053761

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.