ArticleMicrobial cell factories2018
Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 220 citations in OpenAlex.
- Microbial Lignin Valorization to Protocatechuic Acid and Catechol: Biofunneling Pathways and Metabolic Engineering Strategies.Biomolecules · 2026Review
- Engineering a robust and autonomous biological funneling for efficient valorization of lignin-related aromatics.Nature communications · 2026Article
- High-copy plasmid engineering enhances recombinant protein and antimicrobial peptide production in Corynebacterium glutamicum.Microbial cell factories · 2026Article
- Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer.Microbial cell factories · 2026Article
- Engineered microbes to enable a circular economy for biodegradable plastics.Frontiers in microbiology · 2026Review
- Upcycling waste polystyrene to adipic acid through a hybrid chemical and biological process.Nature communications · 2025Article
- Obtaining PDC and other high-added value products from lignin byJournal of integrative bioinformatics · 2025Article
- The TonB-Dependent Transport System Facilitates the Uptake of Inorganic Metal Mediators in Pseudomonas putida KT2440 in a Bioelectrochemical System.Microbial biotechnology · 2025Article
- Investigating the quaternary structure of a homomultimeric catechol 1,2-dioxygenase: An integrative structural biology study.PloS one · 2025Article
- Mild decarboxylation of neat muconic acid to levulinic acid: a combined experimental and computational mechanistic study.RSC advances · 2024Article
- Metabolic Engineering and Process Intensification for Muconic Acid Production UsingInternational journal of molecular sciences · 2024Article
- Systems biology of electrogenic Pseudomonas putida - multi-omics insights and metabolic engineering for enhanced 2-ketogluconate production.Microbial cell factories · 2024Article
- Biological Upcycling of Plastics Waste.Annual review of chemical and biomolecular engineering · 2024Review
- Carbon-wise utilization of lignin-related compounds by synergistically employing anaerobic and aerobic bacteria.Biotechnology for biofuels and bioproducts · 2024Article
- Biological Valorization of Lignin-Derived Aromatics in Hydrolysate to Protocatechuic Acid by EngineeredMolecules (Basel, Switzerland) · 2024Article
- EngineeringApplied and environmental microbiology · 2024Article
- The chemical logic of enzymatic lignin degradation.Chemical communications (Cambridge, England) · 2024Review
- Biosensors for the detection of chorismate and cis,cis-muconic acid in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2024Article
- Bacterial transformation of lignin: key enzymes and high-value products.Biotechnology for biofuels and bioproducts · 2024Review
- Microbial Upcycling of Depolymerized Lignin into Value-Added Chemicals.Biodesign research · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCis, cis-muconic acid (MA) is a dicarboxylic acid of recognized industrial value. It provides direct access to adipic acid and terephthalic acid, prominent monomers of commercial plastics.
resultsIn the present work, we engineered the soil bacterium Corynebacterium glutamicum into a stable genome-based cell factory for high-level production of bio-based MA from aromatics and lignin hydrolysates. The elimination of muconate cycloisomerase (catB) in the catechol branch of the β-ketoadipate pathway provided a mutant, which accumulated MA at 100% molar yield from catechol, phenol, and benzoic acid, using glucose as additional growth substrate. The production of MA was optimized by constitutive overexpression of catA, which increased the activity of the encoded catechol 1,2-dioxygenase, forming MA from catechol, tenfold. Intracellular levels of catechol were more than 30-fold lower than extracellular levels, minimizing toxicity, but still saturating the high affinity CatA enzyme. In a fed-batch process, the created strain C. glutamicum MA-2 accumulated 85 g L
conclusionsOur findings open the door to valorize lignin, the second most abundant polymer on earth, by metabolically engineered C. glutamicum for industrial production of MA and potentially other chemicals.
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