ArticlePloS one2018
An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Morphological details contribute to neuronal response variability within the same cell type.Frontiers in cellular neuroscience · 2026Article
- Molecular Insights Into the Sensory Adaption of the Cave-Dwelling LeechEcology and evolution · 2025Article
- Acceleration of genome rearrangement in clitellate annelids.bioRxiv : the preprint server for biology · 2024Article
- Neural circuit regulation by identified modulatory projection neurons.Frontiers in neuroscience · 2023Review
- Cell anatomy and network input explain differences within but not between leech touch cells at two different locations.Frontiers in cellular neuroscience · 2023Article
- Spatio-Temporal Changes of Extracellular Matrix (ECM) Stiffness in the Development of the LeechInternational journal of molecular sciences · 2022Article
- A review of the bioeffects of low-intensity focused ultrasound and the benefits of a cellular approach.Frontiers in physiology · 2022Review
- Transcriptional profiling of identified neurons in leech.BMC genomics · 2021Article
- Effects of calcium-activated potassium channel modulators on afterhyperpolarizing potentials in identified motor and mechanosensory neurons of the medicinal leech.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2021Article
- Feeding, hunger, satiety and serotonin in invertebrates.Proceedings. Biological sciences · 2020Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The medicinal leech is one of the most venerated model systems for the study of fundamental nervous system principles, ranging from single-cell excitability to complex sensorimotor integration. Yet, molecular analyses have yet to be extensively applied to complement the rich history of electrophysiological study that this animal has received. Here, we generated the first de novo transcriptome assembly from the entire central nervous system of Hirudo verbana, with the goal of providing a molecular resource, as well as to lay the foundation for a comprehensive discovery of genes fundamentally important for neural function. Our assembly generated 107,704 contigs from over 900 million raw reads. Of these 107,704 contigs, 39,047 (36%) were annotated using NCBI's validated RefSeq sequence database. From this annotated central nervous system transcriptome, we began the process of curating genes related to nervous system function by identifying and characterizing 126 unique ion channel, receptor, transporter, and enzyme contigs. Additionally, we generated sequence counts to estimate the relative abundance of each identified ion channel and receptor contig in the transcriptome through Kallisto mapping. This transcriptome will serve as a valuable community resource for studies investigating the molecular underpinnings of neural function in leech and provide a reference for comparative analyses.
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