Evidence map›Paper›PMID 30027472›Full record

ArticleProbiotics and antimicrobial proteins2019

Screening of the Enterocin-Encoding Genes and Their Genetic Determinism in the Bacteriocinogenic Enterococcus faecium GHB21.

Mohamed Merzoug, Khédidja Mosbahi, Daniel Walker, Nour-Eddine Karam

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In one paragraph

Article in Probiotics and antimicrobial proteins, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Mohamed MerzougLaboratoire de Biologie des Microorganismes et Biotechnologie, BP1524, Oran El Mnaouer, Université d'Oran 1 Ahmed Ben Bella, 31000, Oran, Algeria. merzoug.mohamed1@yahoo.fr.ORCID http://orcid.org/0000-0001-7861-2435
Khédidja MosbahiInstitute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciencesf, University of Glasgow, Glasgow, G12 8QQ, UK.
Daniel WalkerInstitute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciencesf, University of Glasgow, Glasgow, G12 8QQ, UK.
Nour-Eddine KaramLaboratoire de Biologie des Microorganismes et Biotechnologie, BP1524, Oran El Mnaouer, Université d'Oran 1 Ahmed Ben Bella, 31000, Oran, Algeria.
Université Oran 1 Ahmed Ben Bella · DZUniversity of Glasgow · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enterococci are well-known for their ability to produce a variety of antimicrobial peptides called enterocins. Most of these enterocins withstand extreme conditions and are very effective against a broad spectrum of undesirable bacteria including some Gram-negative bacteria. The same enterococci strain can produce multiple enterocins simultaneously. The genetic determinants of these bacteriocins can either be located on plasmids or on bacterial chromosome. Digestion of Enterococcus faecium GHB21 plasmids with various restriction endonucleases suggests the presence of two plasmids named pGHB-21.1 and pGHB-21.2 whose respective sizes are ~ 10.0 kb and ~ 3.3 kb. The screening of enterocin-encoding genes among E. faecium GHB21 genome by PCR followed by amplicon sequencing indicated the presence of three different enterocin structural genes similar to entA, entB, and entP genes previously detected in other E. faecium strains. These enterocin genes were, subsequently, localized on the bacterial chromosome based on PCR-targeted screening using total DNA and plasmids of E. faecium GHB21 as separate templates.

Indexed as

Anti-Bacterial AgentsBacterial ProteinsBacteriocinsBridged-Ring CompoundsEnterococcus faeciumGenetic DeterminismPlasmidsAnti-Bacterial AgentsBacterial ProteinsBacteriocinsBridged-Ring CompoundsenterocinE. faecium GHB21Enterocin genesGenetic determinismPCRPlasmids

Identifiers

PMID30027472
OpenAlexW2882971756

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.