ArticleBiotechnology for biofuels2018
Metabolic engineering of
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
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- Evolutionary formation of melatonin and vitamin D in early life forms: insects take centre stage.Biological reviews of the Cambridge Philosophical Society · 2024Review
- Sustainable biosynthesis of squalene from waste cooking oil by the yeastMetabolic engineering communications · 2024Article
- Combinatorial metabolic engineering ofEngineering microbiology · 2023Article
- Characterization of the Sterol 24-C-Methyltransferase Genes Reveals a Network of Alternative Sterol Biosynthetic Pathways inMicrobiology spectrum · 2023Article
- Harnessing bioengineered microbes as a versatile platform for space nutrition.Nature communications · 2022Review
- Development of a High-Titer Culture Medium for the Production of Cholesterol by EngineeredJournal of microbiology and biotechnology · 2022Article
- Reengineering of 7-dehydrocholesterol biosynthesis inFrontiers in microbiology · 2022Article
- Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast.Bioresources and bioprocessing · 2021Review
- Combined Biosynthetic Pathway Engineering and Storage Pool Expansion for High-Level Production of Ergosterol in IndustrialFrontiers in bioengineering and biotechnology · 2021Article
- Compartmentalized Reconstitution of Post-Frontiers in microbiology · 2021Article
- Bornyl Diphosphate Synthase FromFrontiers in bioengineering and biotechnology · 2021Article
- Article
- Microbial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale.Frontiers in bioengineering and biotechnology · 2020Review
- Boosting the biosynthesis of betulinic acid and related triterpenoids in Yarrowia lipolytica via multimodular metabolic engineering.Microbial cell factories · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background7-Dehydrocholesterol (7-DHC) has attracted increasing attentions due to its great medical value and the enlarging market demand of its ultraviolet-catalyzed product vitamin D
resultsIn order to decouple 7-DHC production with cell growth, inducible GAL promoters was employed to control 7-DHC synthesis. Meanwhile, the precursor pool was increased via overexpressing all the mevalonate (MVA) pathway genes (
conclusionsCombined engineering of the pathway and the host cell was adopted in this study to boost 7-DHC output in the yeast. 7-DHC titer was stepwise improved by 26.9-fold compared with the starting strain. This work not only opens large opportunities to realize downstream de novo synthesis of other steroids, but also highlights the importance of the combinatorial engineering of heterologous pathway and host to obtain microbial overproduction of many other natural products.
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