ArticleScientific reports2018
Metabolic Phenotyping and Strain Characterisation of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients Using Rapid Evaporative Ionisation Mass Spectrometry.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Direct Sampling Mass Spectrometry Analysis for the Assessment of Wounds: A Systematic Review.International wound journal · 2025Pooled it
- Application of Rapid Evaporative Ionization Mass Spectrometry (REIMS) to Identify Antimicrobial Resistance in UropathogenicAnalytical chemistry · 2025Article
- Universal, untargeted detection of bacteria in tissues using metabolomics workflows.Nature communications · 2025Article
- Rapid evaporative ionization mass spectrometry: A survey through 15 years of applications.Journal of separation science · 2024Review
- Article
- HILIC-IM-MS for Simultaneous Lipid and Metabolite Profiling of Bacteria.ACS measurement science au · 2024Article
- Article
- Human Milk from Tandem Feeding Dyads Does Not Differ in Metabolite and Metataxonomic Features When Compared to Single Nursling Dyads under Six Months of Age.Metabolites · 2022Article
- Identifying Clinically Relevant Bacteria Directly from Culture and Clinical Samples with a Handheld Mass Spectrometry Probe.Clinical chemistry · 2022Article
- Biological and clinical significance of quorum sensing alkylquinolones: current analytical and bioanalytical methods for their quantification.Analytical and bioanalytical chemistry · 2021Review
- Rapid Evaporative Ionization Mass Spectrometry: A Review on Its Application to the Red Meat Industry with an Australian Context.Metabolites · 2021Review
- The application of rapid evaporative ionization mass spectrometry in the analysis ofOpen biology · 2020Article
- Untargeted LC-MS Metabolomics Differentiates Between Virulent and Avirulent Clinical Strains ofBiomolecules · 2020Article
- Article
- Ambient Ionization Mass Spectrometry: Recent Developments and Applications.Analytical chemistry · 2019Review
- Pathophysiological Mechanisms of Asthma.Frontiers in pediatrics · 2019Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Rapid evaporative ionisation mass spectrometry (REIMS) is a novel technique for the real-time analysis of biological material. It works by conducting an electrical current through a sample, causing it to rapidly heat and evaporate, with the analyte containing vapour channelled to a mass spectrometer. It was used to characterise the metabolome of 45 Pseudomonas aeruginosa (P. aeruginosa) isolates from cystic fibrosis (CF) patients and compared to 80 non-CF P. aeruginosa. Phospholipids gave the highest signal intensity; 17 rhamnolipids and 18 quorum sensing molecules were detected, demonstrating that REIMS has potential for the study of virulence-related metabolites. P. aeruginosa isolates obtained from respiratory samples showed a higher diversity, which was attributed to the chronic nature of most respiratory infections. The analytical sensitivity of REIMS allowed the detection of a metabolome that could be used to classify individual P. aeruginosa isolates after repeated culturing with 81% accuracy, and an average 83% concordance with multilocus sequence typing. This study underpins the capacities of REIMS as a tool with clinical applications, such as metabolic phenotyping of the important CF pathogen P. aeruginosa, and highlights the potential of metabolic fingerprinting for fine scale characterisation at a sub-species level.
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Registered trials
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