ArticleScientific reports2018
Chronic Electrical Stimulation Promotes the Excitability and Plasticity of ESC-derived Neurons following Glutamate-induced Inhibition In vitro.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Electrical Stimulation of Cells: Drivers, Technology, and Effects.Chemical reviews · 2025Review
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- Modeling seizure networks in neuron-glia cultures using microelectrode arrays.Frontiers in network physiology · 2024Article
- Biohybrid neural interfaces: improving the biological integration of neural implants.Chemical communications (Cambridge, England) · 2023Review
- A Workflow to Produce a Low-Cost In Vitro Platform for the Electric-Field Pacing of Cellularised 3D Porous Scaffolds.ACS biomaterials science & engineering · 2023Article
- A microfluidic perspective on conventional in vitro transcranial direct current stimulation methods.Journal of neuroscience methods · 2023Review
- The interplay of intracellular calcium and zinc ions in response to electric field stimulation in primary rat cortical neuronsFrontiers in cellular neuroscience · 2023Article
- 3D TiACS nano · 2022Article
- The effects of electrical stimulation on glial cell behaviour.BMC biomedical engineering · 2022Review
- Pattern Formation in a Spatially Extended Model of Pacemaker Dynamics in Smooth Muscle Cells.Bulletin of mathematical biology · 2022Article
- Influence of Simulated Deep Brain Stimulation on the Expression of Inflammatory Mediators by Human Central Nervous System Cells In Vitro.Neuromolecular medicine · 2022Article
- Neuron Compatibility and Antioxidant Activity of Barium Titanate and Lithium Niobate Nanoparticles.International journal of molecular sciences · 2022Article
- Diseased, differentiated and difficult: Strategies for improved engineering ofFrontiers in cellular neuroscience · 2022Review
- Review
- EngineeringAPL bioengineering · 2021Article
- Development of an objective index, neural activity score (NAS), reveals neural network ontogeny and treatment effects on microelectrode arrays.Scientific reports · 2021Article
- Current State-of-the-Art and Unresolved Problems in Using Human Induced Pluripotent Stem Cell-Derived Dopamine Neurons for Parkinson's Disease Drug Development.International journal of molecular sciences · 2021Review
- Electrophysiological Analysis of Brain Organoids: Current Approaches and Advancements.Frontiers in neuroscience · 2020Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Functional electrical stimulation (FES) is rapidly gaining traction as a therapeutic tool for mediating the repair and recovery of the injured central nervous system (CNS). However, the underlying mechanisms and impact of these stimulation paradigms at a molecular, cellular and network level remain largely unknown. In this study, we used embryonic stem cell (ESC)-derived neuron and glial co-cultures to investigate network maturation following acute administration of L-glutamate, which is a known mediator of excitotoxicity following CNS injury. We then modulated network maturation using chronic low frequency stimulation (LFS) and direct current stimulation (DCS) protocols. We demonstrated that L-glutamate impaired the rate of maturation of ESC-derived neurons and glia immediately and over a week following acute treatment. The administration of chronic LFS and DCS protocols individually following L-glutamate infusion significantly promoted the excitability of neurons as well as network synchrony, while the combination of LFS/DCS did not. qRT-PCR analysis revealed that LFS and DCS alone significantly up-regulated the expression of excitability and plasticity-related transcripts encoding N-methyl-D-aspartate (NMDA) receptor subunit (NR2A), brain-derived neurotrophic factor (BDNF) and Ras-related protein (RAB3A). In contrast, the simultaneous administration of LFS/DCS down-regulated BDNF and RAB3A expression. Our results demonstrate that LFS and DCS stimulation can modulate network maturation excitability and synchrony following the acute administration of an inhibitory dose of L-glutamate, and upregulate NR2A, BDNF and RAB3A gene expression. Our study also provides a novel framework for investigating the effects of electrical stimulation on neuronal responses and network formation and repair after traumatic brain injury.
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