ArticleEquine veterinary journal2019
Advanced mare age impairs the ability of in vitro-matured oocytes to correctly align chromosomes on the metaphase plate.
Article in Equine veterinary journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 42 citations in OpenAlex.
- Age-related changes in lncRNA expression in sperm.Translational andrology and urology · 2025Article
- Strategic approaches to improve equine breeding and stud farm outcomes.Veterinary world · 2025Review
- Chromosomal aberrations and early mortality in a non-mammalian vertebrate: example from pressure-induced triploid Atlantic salmon.Heredity · 2024Article
- Can Nicotinamide Adenine Dinucleotide (NADAnimals : an open access journal from MDPI · 2024Review
- Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses.Frontiers in endocrinology · 2024Article
- Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression ofInternational journal of molecular sciences · 2023Article
- Relationship between estrus endometrial edema and progesterone production in pregnant mares two weeks after ovulation.BMC veterinary research · 2022Article
- Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass.BMC genomics · 2022Article
- Supplemental Nicotinic Acid Elevates NADAnimals : an open access journal from MDPI · 2022Article
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- The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?Animals : an open access journal from MDPI · 2021Review
- Ethics: use and misuse of assisted reproductive techniques across species.Reproduction & fertility · 2021Article
- Equine maternal aging affects oocyte lipid content, metabolic function and developmental potential.Reproduction (Cambridge, England) · 2021Article
- Compromised MPS1 Activity Induces Multipolar Spindle Formation in Oocytes From Aged Mares: Establishing the Horse as a Natural Animal Model to Study Age-Induced Oocyte Meiotic Spindle Instability.Frontiers in cell and developmental biology · 2021Article
- Article
- Whole genome analysis reveals aneuploidies in early pregnancy loss in the horse.Scientific reports · 2020Article
- Placentation in Equids.Advances in anatomy, embryology, and cell biologyArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundAdvanced mare age is associated with declining fertility and an increased risk of early pregnancy loss. Compromised oocyte quality is probably the primary reason for reduced fertility, but the defects predisposing to embryonic death are unknown. In women, advanced age predisposes to chromosome segregation errors during meiosis, which lead to embryonic aneuploidy and a heightened risk of miscarriage.
objectivesTo evaluate the effect of advanced mare age on chromosome alignment and meiotic spindle morphology in in vitro-matured (IVM) oocytes. STUDY
designMorphometric and morphological analysis.
methodsTo investigate differences in spindle organisation and chromosome alignment between young and old mares, oocytes collected from slaughtered mares were divided into two groups depending on mare age (young, ≤14 years and old, ≥16 years), IVM and stained to visualise chromatin and alpha-tubulin. Spindle morphology, morphometry and chromosome (mis)alignment were evaluated by confocal microscopy and 3D image analysis.
resultsOocytes from old mares showed a higher incidence of chromosome misalignment (47.4% vs. 4.5%; P<0.001) and a thicker metaphase plate (mean ± s.d.: 5.8 ± 1.0 μm vs. 4.9 ± 0.9 μm; P = 0.04) than oocytes from young mares. Although no differences in spindle morphometry were detected between old and young mares, an increased major spindle axis length was associated with chromosome misalignment (mean ± s.d.: 25.3 ± 6.1 μm vs. 20.8 ± 3.3 μm; P = 0.01) irrespective of age. MAIN LIMITATIONS: The oocytes were IVM and may not exactly reflect chromosome misalignment in vivo.
conclusionsAdvanced mare age predisposes to chromosome misalignment on the metaphase II spindle of IVM oocytes. The compromised ability to correctly align chromosomes presumably predisposes to aneuploidy in resulting embryos and thereby contributes to the age-related decline in fertility and increased incidence of early pregnancy loss. The Summary is available in Portuguese - see Supporting Information.
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