Evidence map›Paper›PMID 30023823›Full record

ArticleACS omega2018

Distance-Matched Tagging Sequence Optimizes Live-Cell Protein Labeling by a Biarsenical Fluorescent Reagent AsCy3_E.

Karen A Hecht, Yijia Xiong, Daniel A Barrack, Nicole R Ford, Guritno Roesijadi, Thomas C Squier

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Karen A HechtPacific Northwest National Laboratory, Marine Biotechnology Group, 1529 West Sequim Road, Sequim, Washington 98382, United States.
Yijia XiongDepartment of Basic Medical Sciences, Western University of Health Sciences, 200 Mullins Drive, Lebanon, Oregon 97355, United States.
Daniel A BarrackDepartment of Basic Medical Sciences, Western University of Health Sciences, 200 Mullins Drive, Lebanon, Oregon 97355, United States.
Nicole R FordPacific Northwest National Laboratory, Marine Biotechnology Group, 1529 West Sequim Road, Sequim, Washington 98382, United States.
Guritno RoesijadiPacific Northwest National Laboratory, Marine Biotechnology Group, 1529 West Sequim Road, Sequim, Washington 98382, United States.
Thomas C SquierDepartment of Basic Medical Sciences, Western University of Health Sciences, 200 Mullins Drive, Lebanon, Oregon 97355, United States.
Western University of Health Sciences · USPacific Northwest National Laboratory · USOregon State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell permeable biarsenical fluorescent dyes built around a cyanine scaffold (AsCy3) create the ability to monitor the structural dynamics of tagged proteins in living cells. To extend the capability of this photostable and bright biarsenical probe to site-specifically label cellular proteins, we have compared the ability of AsCy3 to label two different tagging sequences (i.e., CCKAEAACC and CCKAEAAKAEAAKCC), which were separately engineered onto enhanced green fluorescent proteins (EGFPs) and expressed in

Identifiers

PMID30023823
PMCPMC6044506
OpenAlexW2788065430

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.