ArticleACS omega2018
Enabling Biocatalysis by High-Throughput Protein Engineering Using Droplet Microfluidics Coupled to Mass Spectrometry.
Article in ACS omega, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 113 citations in OpenAlex.
- Automatic recognition of Pd ions in high-resolution mass spectra of multicomponent samples without visible fine isotope structures.Nature communications · 2026Article
- High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.Analytical chemistry · 2026Article
- Utilizing Venturi effect for automated high-throughput droplet-MS from well plates.The Analyst · 2026Article
- Rapid analysis of terpenes produced by fermentation using flow injection analysis coupled to APCI MS.Analytical methods : advancing methods and applications · 2026Article
- A generalized platform for artificial intelligence-powered autonomous enzyme engineering.Nature communications · 2025Article
- A sticky situation - simple method for rapid poissonian encapsulation of highly aggregation-prone microbeads in polydisperse emulsions.Frontiers in bioengineering and biotechnology · 2025Article
- Coupling of droplet-on-demand microfluidcs with ESI/MS to study single-cell catalysis.RSC advances · 2024Article
- Microdialysis coupled with droplet microfluidics and mass spectrometry for determination of neurotransmittersThe Analyst · 2024Article
- Towards Small Scale: Overview and Applications of Microfluidics in Biotechnology.Molecular biotechnology · 2024Review
- Recent advances in droplet sequential monitoring methods for droplet sorting.Biomicrofluidics · 2023Review
- Mass-Activated Droplet Sorting for the Selection of Lysine-ProducingAnalytical chemistry · 2023Article
- Review
- Non-Native Site-Selective Enzyme Catalysis.Chemical reviews · 2023Review
- Evaluation of Analyte Transfer between Microfluidic Droplets by Mass Spectrometry.Analytical chemistry · 2023Article
- Progresses in Cell-Free In Vitro Evolution.Advances in biochemical engineering/biotechnology · 2023Review
- Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry.Analytical chemistry · 2022Article
- Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis.Nature communications · 2022Article
- Analysis of double-emulsion droplets with ESI mass spectrometry for monitoring lipase-catalyzed ester hydrolysis at nanoliter scale.Analytical and bioanalytical chemistry · 2022Article
- Machine learning for microfluidic design and control.Lab on a chip · 2022Review
- Quantification of Biocatalytic Transformations by Single Microbial Cells Enabled by Tailored Integration of Droplet Microfluidics and Mass Spectrometry.Angewandte Chemie (International ed. in English) · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Directed Evolution is a key technology driving the utility of biocatalysis in pharmaceutical synthesis. Conventional approaches to Directed Evolution are conducted using bacterial cells expressing enzymes in microplates, with catalyzed reactions measured by HPLC, high-performance liquid chromatography-mass spectrometry (HPLC-MS), or optical detectors, which require either long cycle times or tailor-made substrates. To better fit modern, fast-paced process chemistry development where solutions are rapidly needed for new substrates, droplet microfluidics interfaced with electrospray ionization (ESI)-MS provides a label-free high-throughput screening platform. To apply this method to industrial enzyme screening and to explore potential approaches that may further improve the overall throughput, we optimized the existing droplet-MS methods. Carryover between droplets, traditionally a significant issue, was reduced to undetectable level by replacing the stainless steel ESI needle with a Teflon needle within a capillary electrophoresis (CE)-MS source. Throughput was improved to 3 Hz with a wide range of droplet sizes (10-50 nL) by tuning the sheath flow within the CE-MS source. The optimized method was demonstrated by screening reactions using two different transaminase libraries. Good correlations (
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.