ArticleACS omega2017
Quantification of the Disaccharide Trehalose from Biological Samples: A Comparison of Analytical Methods.
Article in ACS omega, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Microbial biopriming and germination cooperatively remodel brown lentil seeds (Lens culinaris L.) metabolome and antidiabetic functionality.NPJ science of food · 2026Article
- Insights into trehalose synthase function in the rhizobiumSustainable microbiology · 2026Article
- Inducible chromosomal rearrangement reveals nonlinear polygenic dosage effects in driving aneuploid yeast traits.Nature communications · 2025Article
- Transcriptome Analysis Reveals the Role of Trehalose in Response to Polyethylene Terephthalate Nanoplastics Treatment in Foxtail Millet (Food science & nutrition · 2025Article
- Engineering a Microfluidic Platform to Cryopreserve Stem Cells: A DMSO-Free Sustainable Approach.Advanced healthcare materials · 2024Article
- The sensor histidine kinase (SLN1) and acetyl-CoA carboxylase (ACC1) coordinately regulate the response ofApplied and environmental microbiology · 2023Article
- Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics.Molecules (Basel, Switzerland) · 2023Article
- Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- Role of Trehalose Synthesis in Ralstonia syzygii subsp. indonesiensis PW1001 in Inducing Hypersensitive Response on Eggplant (Solanum melongena cv. Senryo-nigou).The plant pathology journal · 2021Article
- The putative sensor histidine kinase VadJ coordinates development and sterigmatocystin production in Aspergillus nidulans.Journal of microbiology (Seoul, Korea) · 2021Article
- Parallel evolution of trehalose production machinery in anhydrobiotic animals via recurrent gene loss and horizontal transfer.Open biology · 2021Article
- Conservation of the glycogen metabolism pathway underlines a pivotal function of storage polysaccharides in Chlamydiae.Communications biology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trehalose is a disaccharide that is biosynthesized by many different organisms subjected to extreme conditions, such as dehydration, heat, oxidative stress, and freezing. This disaccharide allows organisms to better survive these environmental stresses; however, the mechanisms by which trehalose exerts its protective effects are not well understood. Methods to accurately measure trehalose from different organisms will help us gain better understanding of these protective mechanisms. In this study, three experimental approaches for the quantification of trehalose from biological samples were compared: an enzymatic trehalose assay (Trehalose Assay Kit; Megazyme International), a high-performance liquid chromatography coupled with refractive index detection-based assay, and a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assay. Limits of detection and quantification for each assay were compared, as were the dynamic ranges for all three assays. The percent recoveries for known amounts of trehalose spiked into bacterial and mammalian cellular lysates were also determined for each of the assays. Finally, endogenous trehalose produced by
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.