ArticleMethodsX2018
Quantification of sugars and organic acids in tomato fruits.
Article in MethodsX, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 135 citations in OpenAlex.
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- Expanding the horizons of levan: from microbial biosynthesis to applications and advanced detection methods.World journal of microbiology & biotechnology · 2024Review
- A Comparative Analysis of XGBoost and Neural Network Models for Predicting Some Tomato Fruit Quality Traits from Environmental and Meteorological Data.Plants (Basel, Switzerland) · 2024Article
- The Effect of Fruit and Berry Pomaces on the Growth Dynamics of Microorganisms and Sensory Properties of Marinated Rainbow Trout.Microorganisms · 2023Article
- Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves-Optimization by a Mixture Design of the Experimental Method.Molecules (Basel, Switzerland) · 2023Article
- Valorisation of Wasted Immature Tomato to Innovative Fermented Functional Foods.Foods (Basel, Switzerland) · 2023Article
- Targeted approaches to improve tomato fruit taste.Horticulture research · 2023Article
- Additive effects of light and branching on fruit size and chemical fruit quality of greenhouse tomatoes.Frontiers in plant science · 2023Article
- Influence of Stirring Parameters on Creaminess of Spring Blossom Honey Measured by Crystal Size, Whiteness Index and Mouthfeel.Foods (Basel, Switzerland) · 2022Article
- Application of Exogenous Melatonin Improves Tomato Fruit Quality by Promoting the Accumulation of Primary and Secondary Metabolites.Foods (Basel, Switzerland) · 2022Article
- Characterization of levansucrase produced by novelHeliyon · 2022Article
- Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sugar and organic acid contents are major factors for tomato fruit flavour and are important breeding traits. Here we provide an improved protocol for accurate quantification of the main sugars, glucose and fructose, and the organic acids, citric acid and malic acid, present in tomato. The tomato extract is spiked with lactose and tricarballylic acid as internal standards and loaded onto a NH2 solid phase extraction (SPE) column. The sugars appear in the flow-through and are subsequently analysed by HPLC using a Nucleodur NH2 column and a refractive index detector. The organic acids bind to the SPE column and are eluted with 400 mM phosphoric acid. For analysis, the organic acids are separated by HPLC using a Nucleodur C18ec column and detected by UV absorption at 210 nm. The method shows excellent inter-day and intra-day reproducibility for glucose, fructose and citric acid with standard deviations of 1-5%. Quantification of citric acid by HPLC and GC-MS showed perfect agreement with a deviation of less than 3%. •Simple method for quantification of glucose, fructose, citric acid and malic acid in tomato.•Efficient removal of interfering compounds by solid phase extraction.•High intra and inter-day reproducibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.