ArticleJournal of microscopy and ultrastructure
Melanogenic effect of purified mushroom tyrosinase on B16F10 Melanocytes: A Phase Contrast and Immunofluorescence Microscopic Study.
Article in Journal of microscopy and ultrastructure. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 11 citations in OpenAlex.
- Tyrosinase Cross-Linked PEG Hydrogels with DAT and DATT as Artificial Substrates: Design, Structure, and Functions.Biomacromolecules · 2026Article
- Purified Mushroom Tyrosinase Induced Melanogenic Protein Expression in B16F10 Melanocytes: A Quantitative Densitometric Analysis.The open medicinal chemistry journal · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cutaneous melanin pigment plays a critical role in camouflage, mimicry, social communication, and protection against harmful effects of solar radiation. It has been proven that tyrosinase plays a pivotal role in melanocytes dendrite formation; however, the molecular mechanism underlying this process has not been fully elucidated. The morphological changes were observed under a phase contrast microscope. These changes were evident, with globular cell bodies and increased numbers of tree branch-like dendrites. The present work aimed to study the morphoanatomic effects of purified tyrosinase to determine its skin-darkening potential using B16F10 melanocyte, which has not been done to date. Phase contrast and immunofluorescence microscopic analysis of B16F10 melanocytes has been done after treatment with various concentrations of purified tyrosinase along with standard tyrosinase (Sigma) in order to explore the mechanism of action of purified tyrosinase induced skin darkening. The phase contrast microscopic results showed that the number of melanocytes with melanin-loaded dendrites has increased significantly in purified tyrosinase treated cells in a dose dependent manner leading to skin darkening. In addition, immunofluorescence microscopic analysis revealed purified tyrosinase increase cellular tyrosinase expression in doze dependent manner due to tyrosinase absorption in B16F10 melanocyte. Present findings proved that purified tyrosinase possesses a skin darkening potential and could be used as a safe melanogenic agent for the treatment of hypopigmentation disorders or vitiligo.
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Registered trials
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