ArticleJournal of translational medicine2018
Platelet storage induces accelerated desialylation of platelets and increases hepatic thrombopoietin production.
Article in Journal of translational medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Clinical application of a modified platelet desialylation test for mechanistic characterization of platelet transfusion refractoriness.Transfusion · 2026Article
- The glycan fingerprint in immune thrombocytopenia.Research and practice in thrombosis and haemostasis · 2026Article
- Design and Characterization of Aromatic Copolyesters Containing Furan and Isophthalic Rings with Suitable Properties for Vascular Tissue Engineering.International journal of molecular sciences · 2025Article
- In vitro Generated Megakaryocytes for the Detection of Human Platelet Antigen-Specific Alloantibodies.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2025Article
- In vitro Hemostatic Functions of Cold-Stored Platelets.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2024Review
- Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect againstJournal of functional biomaterials · 2024Article
- Inhibition of GPIb-α-mediated apoptosis signaling enables cold storage of platelets.Haematologica · 2023Article
- Characterization of the human platelet N- and O-glycome upon storage using tandem mass spectrometry.Blood advances · 2023Article
- Dose-dependent effect of canine lyophilized platelet on an in vitro hemodilution model.BMC veterinary research · 2023Article
- Article
- MicroRNA 320a and Membrane Antigens as Tools to Evaluate the Pathophysiology of Platelets Stored in Blood Banks.Current issues in molecular biology · 2022Article
- Trehalose An Additive Solution for Platelet Concentrate to Protect Platelets from Apoptosis and Clearance during Their Storage at 4°C.Cell journal · 2022Article
- Effects of pre-partum dietary crude protein level on colostrum fat globule membrane proteins and the performance of Hu ewes and their offspring.Frontiers in veterinary science · 2022Article
- Autophagy Ameliorates Reactive Oxygen Species-Induced Platelet Storage Lesions.Oxidative medicine and cellular longevity · 2022Article
- Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia.Frontiers in immunology · 2022Article
- Platelet desialylation and TFH cells-the novel pathway of immune thrombocytopenia.Experimental hematology & oncology · 2021Review
- Platelets as a prognostic marker for sepsis: A cohort study from the MIMIC-III database.Medicine · 2020Article
- Computational Identification and Characterization of New microRNAs in Human Platelets Stored in a Blood Bank.Biomolecules · 2020Article
- Macrophage galactose lectin is critical for Kupffer cells to clear aged platelets.The Journal of experimental medicine · 2020Article
- MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks-A Review.Cells · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStored platelets undergo deleterious changes, referred to as platelet storage lesions (PSLs), which accelerate the desialylation of platelets and result in their phagocytosis and clearance by hepatic macrophages. Recent studies have reported that Ashwell-Morell receptor binds to desialylated platelets, thereby inducing hepatic thrombopoietin (TPO) production in a mouse model. Therefore, this study aimed to demonstrate these relationships between PSL and hepatic TPO production in human study.
methodsPlatelet concentrates (PCs) were obtained from 5 healthy volunteers and the remaining were discarded samples from the blood bank. PCs were divided into two halves, and stored either at 22 or 4 °C. Experiments were conducted using serial samples. Desialylation was assessed using flow cytometry, and structural changes were visualized using electron microscopy. Following co-culture of HepG2 cells (HB-8065, ATCC) with isolated platelets, hepatic TPO production was determined using real-time quantitative polymerase chain reaction and the supernatant TPO level was measured using a Luminex kit.
resultsFor 5 days of storage duration, platelet counts were not influenced by the storage conditions, but the degree of desialylation was proportional to the storage duration. Significant changes in the platelet surface and structure according to storage conditions were noted in electron microscopy. HepG2 cells incubated with aged platelets expressed more TPO mRNA, and supernatant TPO levels were proportional to the storage duration. Refrigeration also influenced on the results of this study, but they were not statistically significant.
conclusionsThis is the first study to demonstrate that, in vitro, aging and refrigeration affect the integrity of human platelets, resulting in induction of hepatic TPO mRNA and protein expression.
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