Evidence map›Paper›PMID 30020957›Full record

ArticlePloS one2018

A novel method for quantifying axon degeneration.

Aaron D Johnstone, Robin M Hallett, Andrés de Léon, Bruno Carturan, Julien Gibon, Philip A Barker

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Aaron D JohnstoneDepartment of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
Robin M HallettThe Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
Andrés de LéonDepartment of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
Bruno CarturanDepartment of Biology, University of British Columbia Okanagan, Kelowna, British Columbia, Canada.
Julien GibonDepartment of Biology, University of British Columbia Okanagan, Kelowna, British Columbia, Canada.
Philip A BarkerDepartment of Biology, University of British Columbia Okanagan, Kelowna, British Columbia, Canada.ORCID 0000-0001-7873-8476
University of British Columbia · CAMontreal Neurological Institute and Hospital · CAUniversity of Toronto · CA

Funding

Canadian Institute of Health Research MOP137057
6 · The paper itself

Abstract

Axons normally degenerate during development of the mammalian nervous system, but dysregulation of the same genetically-encoded destructive cellular machinery can destroy crucial structures during adult neurodegenerative diseases. Nerve growth factor (NGF) withdrawal from dorsal root ganglia (DRG) axons is a well-established in vitro experimental model for biochemical and cell biological studies of developmental degeneration. Definitive methods for measuring axon degeneration have been lacking and here we report a novel method of axon degeneration quantification from bulk cultures of DRG that enables objective and automated measurement of axonal density over the entire field of radial axon outgrowth from the ganglion. As proof of principal, this new method, written as an R script called Axoquant 2.0, was used to examine the role of extracellular Ca2+ in the execution of cytoskeletal disassembly during degeneration of NGF-deprived DRG axons. This method can be easily applied to examine degenerative or neuroprotective effects of gene manipulations and pharmacological interventions.

Indexed as

AxonsBiomarkersCalciumCalcium Chelating AgentsCells, CulturedEgtazic AcidFluorescent Antibody TechniqueGanglia, SpinalImmunohistochemistryMolecular ImagingNerve Growth FactorNeuronsTubulinBiomarkersCalciumCalcium Chelating AgentsEgtazic AcidNerve Growth FactorTubulin

Identifiers

PMID30020957
PMCPMC6051587
OpenAlexW2883902591

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.