ArticlePeerJ2018
'We like it wet': a comparison between dissection techniques for the assessment of parity in
Article in PeerJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- How reliable is the use of ovarian dilatations for mosquito age-grading? A blinded multi-rater validation of the Polovodova method in Anopheles mosquitoes.Journal of medical entomology · 2026Article
- Rapid classification of epidemiologically relevant age categories of the malaria vector, Anopheles funestus.Parasites & vectors · 2024Article
- Article
- Relationships between biological age, distance from aquatic habitats and pyrethroid resistance status of Anopheles funestus mosquitoes in south-eastern Tanzania.Malaria journal · 2022Article
- Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary ofFrontiers in cellular and infection microbiology · 2022Article
- Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium.Microbiome · 2021Review
- Novel sampling methods for monitoringPeerJ · 2021Article
- Fipronil and ivermectin treatment of cattle reduced the survival and ovarian development of field-collected Anopheles albimanus in a pilot trial conducted in northern Belize.Malaria journal · 2019Article
- Autodissemination of pyriproxyfen suppresses stable populations of Anopheles arabiensis under semi-controlled settings.Malaria journal · 2019Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe determination of parous rates in mosquitoes, despite numerous shortcomings, remains a tool to evaluate the effectiveness of control programs and to determine vectorial capacity in malaria vectors. Two dissection techniques are used for this. For one, the tracheoles of dried ovaries are examined with a compound microscope and in the other the follicular stalk of ovaries is examined, wet, with a stereomicroscope. The second method also enables the sac stage of parous insects (which provides information on the duration of the oviposition cycle) and the mated status of insects to be determined. Despite widespread use the two techniques have not previously been compared.
methodsWe compared the two dissection techniques using
resultsSeven per cent of the dry ovaries could not be classified due to granulation (yolk) in the ovariole that obscured the tracheoles. The sensitivity of the dry dissection was 88.51% (CI [79.88-94.35%]) and the specificity was 93.55% (CI [87.68-97.17%]) among the 211 ovaries that could be classified by the dry technique and compared to the ovaries dissected wet. 1,823 live and 1,416 dead from Furvela tent-traps, CDC light-trap and window-trap collections were dissected 'wet' from Tanzania. In these collections parous insects were more likely to die compared to nulliparous ones. The proportion of parous mosquitoes with 'a' sacs (indicative of recent oviposition) was significantly greater in insects that were dead (0.36) on collection in the morning compared to those that were alive (0.12) (Chi square 138.93,
conclusionsIn anophelines the examination of mosquito ovaries using transmitted light in a 'wet' dissection is a more useful and informative technique than examination of dry ovaries. In order to correctly estimate the duration of the oviposition cycle mosquitoes should be dissected as soon as possible after collection. Younger insects were more likely to attempt to feed outdoors rather than indoors.
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