ArticleScientific reports2018
Improved G-AgarTrap: A highly efficient transformation method for intact gemmalings of the liverwort Marchantia polymorpha.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 60 citations in OpenAlex.
- A key role for S-nitrosylation in immune regulation and development in the liverwort Marchantia polymorpha.Journal of experimental botany · 2026Article
- Transcriptomic and metabolomic profiling to unravel sex-dependent mechanisms and the role of exogenous glutathione in Marchantia polymorpha under cadmium stress.BMC plant biology · 2026Article
- MarpolBase: genome database for Marchantia polymorpha featuring high quality reference genome sequences.Plant & cell physiology · 2026Article
- Optimization of selection agent concentrations and expanding G418 utility for gentamicin resistance in Marchantia polymorpha.Scientific reports · 2025Article
- Evolutionary-conserved RLF, a cytochrome bThe New phytologist · 2025Article
- Shikimate Kinase-Like 1 Participates in an Ancient and Conserved Role Contributing to Chloroplast Biogenesis in Land Plants.Molecular biology and evolution · 2025Article
- A Simple and Scalable Chopped-Thallus Transformation Method forPlants (Basel, Switzerland) · 2025Article
- An efficient sulfadiazine selection scheme for stable transformation in the model liverwort Marchantia polymorpha.Journal of experimental botany · 2024Article
- A non-canonical BZR/BES transcription factor regulates the development of haploid reproductive organs in Marchantia polymorpha.Nature plants · 2024Article
- Growth control of Marchantia polymorpha gemmae using nonthermal plasma irradiation.Scientific reports · 2024Article
- Analysis ofFrontiers in plant science · 2024Review
- Parallel evolution of two AIM24 protein subfamilies and their conserved functions in ER stress tolerance in land plants.Plant communications · 2023Article
- Stomatal regulators are co-opted for seta development in the astomatous liverwort Marchantia polymorpha.Nature plants · 2023Article
- Selection of a histidine auxotrophicPlant biotechnology (Tokyo, Japan) · 2022Article
- Polycomb-mediated repression of paternal chromosomes maintains haploid dosage in diploid embryos ofeLife · 2022Article
- The localization of phototropin to the plasma membrane defines a cold-sensing compartment inPNAS nexus · 2022Article
- A glycogen synthase kinase 3-like kinase MpGSK regulates cell differentiation inPlant biotechnology (Tokyo, Japan) · 2022Article
- Measuring and Perturbing Ferroptosis in Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Transformation ofInternational journal of molecular sciences · 2020Article
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4 authors at 2 institutions in 1 country.
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Abstract
Liverworts are key species for studies of plant evolution, occupying a basal position among the land plants. Marchantia polymorpha has emerged as a highly studied model liverwort, and many relevant techniques, including genetic transformation, have been established for this species. Agrobacterium-mediated transformation is widely used in many plant species because of its low cost. Recently, we developed a simplified Agrobacterium-mediated method for transforming M. polymorpha, known as AgarTrap (agar-utilized transformation with pouring solutions). The AgarTrap procedure, which involves culturing the liverwort tissue in various solutions on a single solid medium, yields up to a hundred independent transformants. AgarTrap is a simple procedure, requiring minimal expertise, cost, and time. Here, we investigated four factors that influence AgarTrap transformation efficiency: (1) humidity, (2) surfactant in the transformation buffer, (3) Agrobacterium strain, and (4) light/dark condition. We adapted the AgarTrap protocol for transforming intact gemmalings, achieving an exceptionally high transformation efficiency of 97%. The improved AgarTrap method will enhance the molecular biological study of M. polymorpha. Furthermore, this method provides new possibilities for improving transformation techniques for a variety of plant species.
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