Evidence map›Paper›PMID 30018113›Full record

ArticlemBio2018

Structure-Function Analysis of the Curli Accessory Protein CsgE Defines Surfaces Essential for Coordinating Amyloid Fiber Formation.

Roger D Klein, Qin Shu, Zachary T Cusumano, Kanna Nagamatsu, Nathaniel C Gualberto, Aaron J L Lynch, Chao Wu, Wenjie Wang, Neha Jain, Jerome S Pinkner and 4 more

Open access · goldAbstract read
In one paragraph

Article in mBio, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 44 citations in OpenAlex.

  1. Review
  2. Article
  3. CRISPRi Screening Identifies EssentialInternational journal of molecular sciences · 2026
    Article
  4. Article
  5. Genomic profiling of ESBL/AmpC-producingFrontiers in microbiology · 2026
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Antibiofilm properties of cathelicidin LL-37: an in-depth review.World journal of microbiology & biotechnology · 2023
    Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Roger D Klein *Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Qin Shu *Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, USA.
Zachary T CusumanoDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Kanna NagamatsuDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA.
Nathaniel C GualbertoDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Aaron J L LynchDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Chao WuDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
Wenjie WangDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
Neha JainDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA.
Jerome S PinknerDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Gaya K AmarasingheDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
Scott J HultgrenDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA hultgren@wustl.edu frieden@wustl.edu chapmanm@umich.edu.
Carl FriedenDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, USA hultgren@wustl.edu frieden@wustl.edu chapmanm@umich.edu.
Matthew R ChapmanDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA hultgren@wustl.edu frieden@wustl.edu chapmanm@umich.edu.
Washington University in St. Louis · USUniversity of Michigan–Ann Arbor · US

Funding

NATIONAL RESEARCH SERVICE AWARD-MEDICAL SCIENTISTT32GM007200 · NIGMS · WASHINGTON UNIVERSITY · PI YOKOYAMA, WAYNE M. · 1985 to 2024
$59.8M
Virology BSL4 CoreP01AI120943 · NIAID · WASHINGTON UNIVERSITY · PI Gaya K. Amarasinghe · 2016 to 2026
$30.2M
Protein and Chemical Modulation of Curli Amyloid BiogenesisR01GM118651 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Matthew Richard Chapman · 2016 to 2026
$3.0M
BIOGENESIS AND INHIBITION OF BIOFILM-ASSOCIATED BACTERIAL AMYLOIDR01AI099099 · NIAID · WASHINGTON UNIVERSITY · PI FRIEDEN, CARL, HULTGREN, SCOTT J. · 2013 to 2017
$1.9M
NIAID NIH HHS P01 AI120943NIAID NIH HHS R01 AI099099NIGMS NIH HHS R01 GM118651NIGMS NIH HHS T32 GM007200
6 · The paper itself

Abstract

Curli amyloid fibers are produced as part of the extracellular biofilm matrix and are composed primarily of the major structural subunit CsgA. The CsgE chaperone facilitates the secretion of CsgA through CsgG by forming a cap at the base of the nonameric CsgG outer membrane pore. We elucidated a series of finely tuned nonpolar and charge-charge interactions that facilitate the oligomerization of CsgE and its ability to transport unfolded CsgA to CsgG for translocation. CsgE oligomerization

Indexed as

AmyloidBiofilmsEscherichia coliEscherichia coli ProteinsExtracellular Polymeric Substance MatrixLipoproteinsMembrane Transport ProteinsModels, BiologicalModels, MolecularMutationPolymerizationProtein BindingProtein ConformationProtein StabilityAmyloidcsgA protein, E coliCsgE protein, E coliCsgG protein, E coliEscherichia coli ProteinsLipoproteinsMembrane Transport ProteinsbioassemblybiofilmscurliEscherichia coliextracellular matrixfunctional amyloidnuclear magnetic resonancenucleation-precipitation

Identifiers

PMID30018113
PMCPMC6050966
OpenAlexW2883790338

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.