ArticleMolecular therapy : the journal of the American Society of Gene Therapy2018
Ancestral Adeno-Associated Virus Vector Delivery of Opsins to Spiral Ganglion Neurons: Implications for Optogenetic Cochlear Implants.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- Delivery of AAV vectors to the superior olivary complex enables efficient adult cochlear transduction via axonal transport.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Targeted spiral ganglion neuron degeneration in parvalbumin-Cre neonatal mice.Molecular therapy. Methods & clinical development · 2025Article
- Toward elucidating the mechanism of cochlear stimulation by infrared light.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors.Cell proliferation · 2025Article
- Inner Ear Gene Therapy: An Overview from Bench to Bedside.Molecular diagnosis & therapy · 2025Review
- Study on Recovery Strategy of Hearing Loss & SGN Regeneration Under Physical Regulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Improved optogenetic modification of spiral ganglion neurons for future optical cochlear implants.Theranostics · 2025Article
- Combined-electrical optogenetic stimulation but not channelrhodopsin kinetics improves the fidelity of high rate stimulation in the auditory pathway in mice.Scientific reports · 2024Article
- Emerging optogenetics technologies in biomedical applications.Smart medicine · 2023Review
- Review
- Channelrhodopsin fluorescent tag replacement for clinical translation of optogenetic hearing restoration.Molecular therapy. Methods & clinical development · 2023Article
- Towards translational optogenetics.Nature biomedical engineering · 2023Review
- Signal processing and stimulation potential within the ascending auditory pathway: a review.Frontiers in neuroscience · 2023Review
- Patient perspectives on the need for improved hearing rehabilitation: A qualitative survey study of German cochlear implant users.Frontiers in neuroscience · 2023Article
- Is there an unmet medical need for improved hearing restoration?EMBO molecular medicine · 2022Review
- Analyzing efficacy, stability, and safety of AAV-mediated optogenetic hearing restoration in mice.Life science alliance · 2022Article
- PRDX1 activates autophagy via the PTEN-AKT signaling pathway to protect against cisplatin-induced spiral ganglion neuron damage.Autophagy · 2021Article
- Utility of red-light ultrafast optogenetic stimulation of the auditory pathway.EMBO molecular medicine · 2021Article
- Viral-mediated transduction of auditory neurons with opsins for optical and hybrid activation.Scientific reports · 2021Article
- Multiscale photonic imaging of the native and implanted cochlea.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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11 authors at 3 institutions in 1 country.
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Abstract
Optogenetics is a transformative technology based on light-sensitive microbial proteins, known as opsins, that enable precise modulation of neuronal activity with pulsed radiant energy. Optogenetics has been proposed as a means to improve auditory implant outcomes by reducing channel interaction and increasing electrode density, but the introduction of opsins into cochlear spiral ganglion neurons (SGNs) in vivo has been challenging. Here we test opsin delivery using a synthetically developed ancestral adeno-associated virus (AAV) vector called Anc80L65. Wild-type C57BL/6 mouse pups were injected via the round window of cochlea with Anc80L65 carrying opsin Chronos under the control of a CAG promoter. Following an incubation of 6-22 weeks, pulsed blue light was delivered to cochlear SGNs via a cochleosotomy approach and flexible optical fiber. Optically evoked auditory brainstem responses (oABRs) and multiunit activity in inferior colliculus (IC) were observed. Post-experiment cochlear histology demonstrated opsin expression in SGNs (mean = 74%), with an even distribution of opsin along the cochlear basal/apical gradient. This study is the first to describe robust SGN transduction, opsin expression, and optically evoked auditory electrophysiology in neonatal mice. Ultimately, this work may provide the basis for a new generation of cochlear implant based on light.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.