ArticleJournal of structural biology2018
A simple and robust procedure for preparing graphene-oxide cryo-EM grids.
Article in Journal of structural biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
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Who cites it
77 citing papers in PubMed.
- Structural and genetic dissection of RNA-guided gene repression by TnpB-derived transcription factors.bioRxiv : the preprint server for biology · 2026Article
- De novo design of semisynthetic conduction pores.Nature structural & molecular biology · 2026Article
- Core nucleosomes are refractory to lentiviral DNA integration.Nature communications · 2026Article
- WebCalEM: a browser-based tool for routine and accurate pixel-size calibration in cryo-EM.Acta crystallographica. Section F, Structural biology communications · 2026Article
- WebCalEM: a browser-based tool for routine and accurate pixel size calibration in cryo-EM.bioRxiv : the preprint server for biology · 2026Article
- Strategic advances for cryo-EM structural studies of small (<100 kDa) GPCRs.Communications biology · 2026Review
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Metabolic environment-driven remodeling of mitochondrial ribosomes regulates translation and biogenesis.Molecular cell · 2025Article
- The mechanism of pathogenic αProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The HIV-1 nuclear export complex reveals the role of RNA in CRM1 cargo recognition.Molecular cell · 2025Article
- Review
- Autoinhibition imposed by a large conformational switch of INO80 regulates nucleosome positioning.Science (New York, N.Y.) · 2025Article
- Structure of the human autophagy factor EPG5 and the molecular basis of its conserved mode of interaction with Atg8-family proteins.Autophagy · 2025Article
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- Mechanism of mammalian transcriptional repression by noncoding RNA.Nature structural & molecular biology · 2025Article
- Expression and Purification of SARS-related Spike Glycoproteins for Cryo-EM Analysis.Current protocols · 2025Article
- Structural characterization of the ABC transporter DppABCDF in Escherichia coli reveals insights into dipeptide acquisition.PLoS biology · 2025Article
- Cryo-EM structures of apo and atorvastatin-bound human 3-hydroxy-3-methylglutaryl-coenzyme A reductase.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Cryo-Electron Microscopy-Based Epitope Mapping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.Nature communications · 2024Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Graphene oxide (GO) sheets have been used successfully as a supporting substrate film in several recent cryogenic electron-microscopy (cryo-EM) studies of challenging biological macromolecules. However, difficulties in preparing GO-covered holey carbon EM grids have limited their widespread use. Here, we report a simple and robust method for covering holey carbon EM grids with GO sheets and demonstrate that these grids can be used for high-resolution single particle cryo-EM. GO substrates adhere macromolecules, allowing cryo-EM grid preparation with lower specimen concentrations and provide partial protection from the air-water interface. Additionally, the signal of the GO lattice beneath the frozen-hydrated specimen can be discerned in many motion-corrected micrographs, providing a high-resolution fiducial for evaluating beam-induced motion correction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.