ArticleJournal of the American Chemical Society2018
Site-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms.
Article in Journal of the American Chemical Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.bioRxiv : the preprint server for biology · 2026Article
- Automated Covalent Microcontact Bioprinting of Lubricant-Infused Microarrays for Biological Assays.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Stem cell secretome armed magneto-actuated micromotors as spatio-temporal manipulators for wound healing acceleration.Nature communications · 2025Article
- Thio-NHS esters are non-innocent protein acylating reagents.Nature communications · 2025Article
- One-Pot Construction of NHS-Activated Magnetic Particles for Chemoselective Capture of Carboxyl Metabolites.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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- Uncoupling histone modification crosstalk by engineering lysine demethylase LSD1.Nature chemical biology · 2025Article
- Push-pull fluorophores based on NHS esters of bithiophene for labelling of biomolecules containing primary amines.Royal Society open science · 2025Article
- Crossing the Solubility Rubicon: 15-Crown-5 Facilitates the Preparation of Water-Soluble Sulfo-NHS Esters in Organic Solvents.Bioconjugate chemistry · 2024Article
- Recent Advances in Bioorthogonal Ligation and Bioconjugation.Topics in current chemistry (Cham) · 2023Review
- Human Complement Inhibits Myophages againstViruses · 2023Article
- Emerging chemical engineering of exosomes as "bioscaffolds" in diagnostics and therapeutics.Genes & diseases · 2023Review
- Semisynthetic Approach to the Analysis of Tumor Suppressor PTEN Ubiquitination.Journal of the American Chemical Society · 2023Article
- Development and Recent Advances in Lysine and N-Terminal Bioconjugation for Peptides and Proteins.Molecules (Basel, Switzerland) · 2023Review
- Chemical and structural approaches to investigate PTEN function and regulation.Methods in enzymology · 2023Article
- Article
- Enzymatic analysis of WWP2 E3 ubiquitin ligase using protein microarrays identifies autophagy-related substrates.The Journal of biological chemistry · 2022Article
- Utilizing a Baculovirus/Insect Cell Expression System and Expressed Protein Ligation (EPL) for Protein Semisynthesis.Current protocols · 2022Article
- The structural basis of PTEN regulation by multi-site phosphorylation.Nature structural & molecular biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
N-Hydroxysuccinimide (NHS)-esters are widely used to label proteins nonselectively on free amino groups. Such broad labeling can be disadvantageous because it can interfere with protein structure or function and because stoichiometry is poorly controlled. Here we describe a simple method to transform NHS-esters into site-specific protein labeling on N-terminal Cys residues. MESNA addition converts NHS-esters to chemoselective thioesters for N-Cys modification. This labeling strategy was applied to clarify mechanistic features of the ubiquitin E3 ligase WWP2 including its interaction with one of its substrates, the tumor suppressor PTEN, as well as its autoubiquitination molecularity. We propose that this convenient protein labeling strategy will allow for an expanded application of NHS-esters in biochemical investigation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.