ArticleMolecular medicine reports2018
miR‑200b‑3p inhibits proliferation and induces apoptosis in colorectal cancer by targeting Wnt1.
Article in Molecular medicine reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Diagnostic and Therapeutic Potential of Selected microRNAs in Colorectal Cancer: A Literature Review.Cancers · 2025Review
- Mechanisms of apoptosis-related non-coding RNAs in ovarian cancer: a narrative review.Apoptosis : an international journal on programmed cell death · 2025Review
- Exploring miRNA profile associated with cisplatin resistance in ovarian cancer cells.Biochemistry and biophysics reports · 2025Article
- CD44 knockdown alters miRNA expression and their target genes in colon cancer.Frontiers in immunology · 2025Article
- The implication of TET1, miR-200, and miR-494 expression with tumor formation in colorectal cancer: through targeting Wnt signaling.Molecular biology reports · 2024Article
- Article
- miR-200b-3p accelerates progression of pituitary adenomas by negatively regulating expression of RECK.Oncology research · 2024Article
- Loss of exosomal miR-200b-3p from hypoxia cancer-associated fibroblasts promotes tumorigenesis and reduces sensitivity to 5-Flourouracil in colorectal cancer via upregulation of ZEB1 and E2F3.Cancer gene therapy · 2023Article
- Astragalus polysaccharide ameliorates steroid-induced osteonecrosis of the femoral head by regulating miR-200b-3p-mediated Wnt/β-catenin signaling pathway via inhibiting SP1 expression : Astragalus polysaccharide regulates SONFH via SP1.BMC musculoskeletal disorders · 2023Article
- The Roles of Secreted Wnt Ligands in Cancer.International journal of molecular sciences · 2023Review
- MiR-200b-3p is upregulated in the placental tissues from patients with preeclampsia and promotes the development of preeclampsia via targeting profilin 2.Cell cycle (Georgetown, Tex.) · 2022Article
- MicroRNA global profiling in cystic fibrosis cell lines reveals dysregulated pathways related with inflammation, cancer, growth, glucose and lipid metabolism, and fertility: an exploratory study.Acta bio-medica : Atenei Parmensis · 2022Article
- Regulatory functions of miR‑200b‑3p in tumor development (Review).Oncology reports · 2022Review
- MicroRNA-200b-3p restrains gastric cancer cell proliferation, migration, and invasion via C-X-C motif chemokine ligand 12/CXC chemokine receptor 7 axis.Bioengineered · 2022Article
- The role of miR-200 family in the regulation of hallmarks of cancer.Frontiers in oncology · 2022Review
- WNT8B as an Independent Prognostic Marker for Nasopharyngeal Carcinoma.Current oncology (Toronto, Ont.) · 2021Article
- Targeting Signaling Pathway Networks in Several Malignant Tumors: Progresses and Challenges.Frontiers in pharmacology · 2021Review
- The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors.International journal of molecular sciences · 2020Article
- Emerging Roles of Wnt Ligands in Human Colorectal Cancer.Frontiers in oncology · 2020Review
- MicroRNA‑106b functions as an oncogene and regulates tumor viability and metastasis by targeting LARP4B in prostate cancer.Molecular medicine reports · 2019Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
MicroRNA (miR)‑200b‑3p is downregulated in multiple human cancer types. Wnt signaling serves a role in human colorectal cancer (CRC). The present study aimed to examine the effect of miR‑200b‑3p on human CRC and its potential association with Wnt signaling. The Cell Counting Kit‑8 (CCK‑8) was employed to assess cell viability. A flow cytometric assay was conducted to examine cell proliferation and apoptosis. The regulation model of miR‑200b‑3p and Wnt1 was assessed by a luciferase reporter assay. A commercial kit was used to evaluate the activity of caspase‑3 following treatment of the cells by miR‑200b‑3p or Wnt1. The expression of target factors was determined by a quantitative real‑time polymerase chain reaction and western blot analysis. The expression of miR‑200b‑3p was decreased in human CRC tissues and in cell lines. The bioinformatics analysis and the luciferase reporter assay revealed that Wnt1 may be a direct target of miR‑200b‑3p. Moreover, the viability and proliferation of CRC cells was suppressed by miR‑200b‑3p. miR‑200b‑3p additionally induced apoptosis in CRC cells. Furthermore, the caspase‑3 activity was enhanced in the miR‑200b‑3p mimics group. The expression of antigen Ki‑67 (additionally termed KI‑67) and β‑catenin was decreased, while the expression of cleaved caspase‑3 was increased by miR‑200b‑3p. In conclusion, miR‑200b‑3p inhibited proliferation and induced apoptosis in CRC cells by inactivating Wnt/β‑catenin signaling. The present study provided potential biomarkers and candidate modalities for the management of CRC.
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