Evidence map›Paper›PMID 30014964›Full record

ArticleBiomaterials2018

Cellular interactions with hydrogel microfibers synthesized via interfacial tetrazine ligation.

Shuang Liu, Axel C Moore, Aidan B Zerdoum, Han Zhang, Samuel L Scinto, He Zhang, Liang Gong, David L Burris, Ayyappan K Rajasekaran, Joseph M Fox and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Advanced Multifunctional Hydrogels for Enhanced Wound Healing through Ultra-Fast Selenol-SAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Shuang LiuDepartment of Materials Science and Engineering, University of Delaware, Newark, DE 19716, USA.
Axel C MooreDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716, USA.
Aidan B ZerdoumDepartment of Biomedical Engineering, University of Delaware, Newark, DE 19716, USA.
Han ZhangDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
Samuel L ScintoDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
He ZhangDepartment of Materials Science and Engineering, University of Delaware, Newark, DE 19716, USA.
Liang GongDepartment of Materials Science and Engineering, University of Delaware, Newark, DE 19716, USA.
David L BurrisDepartment of Mechanical Engineering, University of Delaware, Newark, DE 19716, USA.
Ayyappan K RajasekaranTherapy Architects, LLC, Helen F Graham Cancer Center Newark, DE 19718, USA.
Joseph M FoxDepartment of Materials Science and Engineering, University of Delaware, Newark, DE 19716, USA; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA. Electronic address: jmfox@udel.edu.
Xinqiao JiaDepartment of Materials Science and Engineering, University of Delaware, Newark, DE 19716, USA; Department of Biomedical Engineering, University of Delaware, Newark, DE 19716, USA. Electronic address: xjia@udel.edu.
University of Delaware · US

Funding

A Hydrogel-Based Cellular Model of the Human Vocal FoldR01DC014461 · NIDCD · UNIVERSITY OF DELAWARE · PI Xinqiao Jia · 2016 to 2026
$6.2M
Pilot Research Subproject ProgramP30GM110758 · NIGMS · UNIVERSITY OF DELAWARE · PI BEEBE, THOMAS PAUL · 2014 to 2018
$5.8M
Functional Assembly of Salivary Cells to Relieve XerostomiaR01DE022969 · NIDCR · RICE UNIVERSITY · PI FARACH-CARSON, MARY C, JIA, XINQIAO · 2012 to 2015
$2.5M
Zeiss LSM710 Inverted Confocal MicroscopeS10OD016361 · OD · UNIVERSITY OF DELAWARE · PI CAPLAN, JEFFREY L · 2015 to 2015
$444k
NIDCD NIH HHS R01 DC014461NIDCR NIH HHS R01 DE022969NIGMS NIH HHS P30 GM110758NIH HHS S10 OD016361
6 · The paper itself

Abstract

Fibrous proteins found in the natural extracellular matrix (ECM) function as host substrates for migration and growth of endogenous cells during wound healing and tissue repair processes. Although various fibrous scaffolds have been developed to recapitulate the microstructures of the native ECM, facile synthesis of hydrogel microfibers that are mechanically robust and biologically active have been elusive. Described herein is the use of interfacial bioorthogonal polymerization to create hydrogel-based microfibrous scaffolds via tetrazine ligation. Combination of a trifunctional strained trans-cyclooctene monomer and a difunctional s-tetrazine monomer at the oil-water interface led to the formation of microfibers that were stable under cell culture conditions. The bioorthogonal nature of the synthesis allows for direct incorporation of tetrazine-conjugated peptides or proteins with site-selectively, genetically encoded tetrazines. The microfibers provide physical guidance and biochemical signals to promote the attachment, division and migration of fibroblasts. Mechanistic investigations revealed that fiber-guided cell migration was both F-actin and microtubule-dependent, confirming contact guidance by the microfibers. Prolonged culture of fibroblasts in the presence of an isolated microfiber resulted in the formation of a multilayered cell sheet wrapping around the fiber core. A fibrous mesh provided a 3D template to promote cell infiltration and tissue-like growth. Overall, the bioorthogonal approach led to the straightforward synthesis of crosslinked hydrogel microfibers that can potentially be used as instructive materials for tissue repair and regeneration.

Indexed as

AnimalsCell Culture TechniquesCell MovementFibroblastsHumansHydrogelsPeptidesPolymerizationProteinsTissue ScaffoldsWound HealingHydrogelsPeptidesProteinsCell migrationContact guidanceHydrogel microfibersInterfacial polymerizationTetrazine ligationWound healing

Identifiers

PMID30014964
PMCPMC6091885
OpenAlexW2844941680

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.