ArticleGastroenterology and hepatology from bed to bench2018
A rapid and sensitive assay to identify HLA-DQ2/8 risk alleles for celiac disease using real-time PCR method.
Article in Gastroenterology and hepatology from bed to bench, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Dietary Intervention for Systemic Lupus Erythematosus patients based on HLA-DQ2 and DQ8 genotyping (DISH): a protocol for a randomised controlled study.Lupus science & medicine · 2025Article
- Evaluating the Correlation Between Fecal Microbiota Profiles and Clinical Presentation in Pediatric Patients with Celiac Disease.Middle East journal of digestive diseases · 2025Article
- A gluten-free diet has a different effect on the iron profile of celiac disease and non-celiac gluten-sensitive patients with idiopathic iron deficiency anaemia.Gastroenterology and hepatology from bed to bench · 2025Article
- Investigating the Impact of Vitamin A and Amino Acids on Immune Responses in Celiac Disease Patients.Diseases (Basel, Switzerland) · 2024Article
- Suppressive Mechanisms Induced by Tregs in Celiac DiseaseIranian biomedical journal · 2020Review
- The role of CXCR3 and its ligands CXCL10 and CXCL11 in the pathogenesis of celiac disease.Medicine · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo perform a simple, rapid and sensitive Real-time PCR based SYBR Green method to determine the human leukocyte antigen (HLA)-DQ 2/8 alleles in celiac disease (CD) patients.
backgroundMany molecular techniques are available to determine the HLA-DQ2 and DQ8 alleles, but they are too expensive and have many steps that make them difficult to use.
methodsTo determine the HLA-DQ 2/8 alleles we have developed a new real-time PCR assay, using SYBR Green technique with melting curve analysis on genomic DNA isolated from 75 CD patients and 94 healthy controls. The specific primers to examine HLA-DQA1*05, HLA-DQB1*02 and HLA-DQB1*0302 alleles were used and results were compared with commercially available kits.
resultsUsing this method, the presence of HLA-DQ2 and HLA-DQ8 alleles were determined with sensitivity and specificity 80% and 100% respectively and compared to low resolution commercially available kits, the results of this method were more efficient. The frequency of DQ2 and DQ8 in patients was 76% and 29%, respectively and overall 96% of patients were carries DQ2 and/or DQ8 alleles.
conclusionThe result of this study showed that Real-time PCR using SYBR Green method with melting curve analysis has good efficiency to identify the HLA-DQ2/8 risk alleles.
Indexed as
Identifiers
30013750PMC6040037W2849862075What OpenQuestion holds
Registered trials
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