ArticleScientific reports2018
Gene expression profiles of germ-free and conventional piglets from the same litter.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Single cell transcriptome profiling of immune tissues from germ-free and specific pathogen-free piglet.Scientific data · 2025Article
- Research Progress on the Preparation and Application of Decellularized Tendons.Current issues in molecular biology · 2025Review
- The role of the gut microbiota in regulating responses to vaccination: current knowledge and future directions.The FEBS journal · 2025Review
- Effects of the commensal microbiota on spleen and mesenteric lymph node immune function: investigation in a germ-free piglet model.Frontiers in microbiology · 2024Article
- Impacts of maternal microbiota and microbial metabolites on fetal intestine, brain, and placenta.BMC biology · 2023Article
- Commensal microbiota regulate renal gene expression in a sex-specific manner.American journal of physiology. Renal physiology · 2023Article
- Commensal microbiota modulates phenotypic characteristics and gene expression in piglet Peyer's patches.Frontiers in physiology · 2023Article
- Intestinal Exposure to Ceftiofur and Cefquinome after Intramuscular Treatment and the Impact of Ceftiofur on the Pig Fecal Microbiome and Resistome.Antibiotics (Basel, Switzerland) · 2022Article
- Metatranscriptomic Analysis of the Chicken Gut Resistome Response to In-Feed Antibiotics and Natural Feed Additives.Frontiers in microbiology · 2022Article
- Integrated analysis of lncRNA-mediated ceRNA network involved in immune regulation in the spleen of Meishan piglets.Frontiers in veterinary science · 2022Article
- Gut microbiota contributes to the methionine metabolism in host.Frontiers in microbiology · 2022Article
- Single-cell sequencing of rotavirus-infected intestinal epithelium reveals cell-type specific epithelial repair and tuft cell infection.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The intestinal microbiota contributes to the growth and physiological state of muscle tissue in piglets.Scientific reports · 2021Article
- Hypochlorous acid-generating electrochemical scaffold eliminates Candida albicans biofilms.Journal of applied microbiology · 2020Article
- Limited influence of the microbiome on the transcriptional profile of female Aedes aegypti mosquitoes.Scientific reports · 2020Article
- Abnormal Food Timing Promotes Alcohol-Associated Dysbiosis and Colon Carcinogenesis Pathways.Frontiers in oncology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germ-free (GF) pigs have clear microbiological backgrounds, and are extensively used as large animal models in the biomedical sciences. However, investigations of the transcriptomic differences between GF and cesarean-derived conventional (CV) piglets are limited. To improve our understanding of GF pigs, and to increase the utility of pigs as an alternative non-rodent model, we used RNA sequencing to profile gene expression in five tissues (the oral mucosae, jejunum, colon, liver, and spleen) of four male GF piglets and four male CV piglets from the same litter. We identified 14 genes that were differentially expressed in all five tissues. Seven of these common differentially expressed genes (DEGs) were interferon-inducible genes, and all 14 were consistently downregulated in the GF piglets as compared to the CV piglets. Compared to the other tissues tested, the expression of transcription factors (TFs) in the colon was most affected by the absence of a microbiota. The expression patterns of immune-related genes were downregulated in the GF piglets as compared to the CV piglets, indicating that the intestinal microbiota influenced gene expression in other tissues besides the gut. Gene Ontology (GO) analysis indicated that, in pigs, the intestinal microbiota affected the expression of genes related to immune system function and development.
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