Evidence map›Paper›PMID 30012865›Full record

ArticleMolecular and cellular biology2018

Identification of a Novel Enhancer/Chromatin Opening Element Associated with High-Level γ-Globin Gene Expression.

Yong Shen, MacLean A Bassett, Aishwarya Gurumurthy, Rukiye Nar, Isaac J Knudson, Cameron R Guy, Alex Perez, Russell W Mellen, Masatoshi Ikeda, Mir A Hossain and 3 more

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. TheJournal of pediatric genetics · 2023
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Yong ShenDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
MacLean A BassettDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Aishwarya GurumurthyDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Rukiye NarDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Isaac J KnudsonDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Cameron R GuyDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Alex PerezDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Russell W MellenDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Masatoshi IkedaDepartment of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
Mir A HossainDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Suming HuangDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA.
Kazuhiko IgarashiDepartment of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
Jörg BungertDepartment of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA jbungert@ufl.edu.
University of Florida Health · USTohoku University · JP

Funding

STRUCTURE/FUNCTION OF THE HUMAN B-GLOBIN LCRR01DK052356 · NIDDK · UNIVERSITY OF FLORIDA · PI BUNGERT, JORG · 1997 to 2017
$4.7M
The Role of Long Noncoding RNA in HematopoiesisR01DK110108 · NIDDK · UNIVERSITY OF FLORIDA · PI HUANG, SUMING · 2016 to 2019
$1.3M
The role of a lincRNA in chromatin structure and hematopoiesisR56DK101994 · NIDDK · UNIVERSITY OF FLORIDA · PI HUANG, SUMING · 2015 to 2015
$120k
NIDDK NIH HHS R01 DK052356NIDDK NIH HHS R01 DK110108NIDDK NIH HHS R56 DK101994
6 · The paper itself

Abstract

The organization of the five β-type globin genes on chromosome 11 reflects the timing of expression during erythroid cell development, with the embryonic ε-globin gene being located at the 5' end, followed by the two fetal γ-globin genes, and with the adult β- and δ-globin genes being located at the 3' end. Here, we functionally characterized a DNase I-hypersensitive site (HS) located 4 kb upstream of the Gγ-globin gene (HBG-4kb HS). This site is occupied by transcription factors USF1, USF2, EGR1, MafK, and NF-E2 in the human erythroleukemia cell line K562 and exhibits histone modifications typical for enhancers. We generated a synthetic zinc finger (ZF) DNA-binding domain targeting the HBG-4kb HS (HBG-4kb ZF). The HBG-4kb ZF interacted with the target site

Indexed as

ChromatinDeoxyribonuclease IEnhancer Elements, GeneticErythropoiesisgamma-GlobinsGene Expression Regulation, DevelopmentalGenes, SwitchHistone CodeHumansK562 CellsModels, GeneticPolymorphism, Single NucleotideRNAChromatinDeoxyribonuclease Igamma-GlobinsRNAchromatinenhancergene regulationglobinhemoglobinhistonessynthetic zinc-finger DNA-binding domaintranscriptionzinc finger DNA-binding domainzinc finger proteins

Identifiers

PMID30012865
PMCPMC6146835
OpenAlexW2884340256

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.