ArticleMolecular systems biology2018
Characterizing meiotic chromosomes' structure and pairing using a designer sequence optimized for Hi-C.
Article in Molecular systems biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- High-resolution chromatin mapping reveals that CTCF anchors meiotic loops to the chromosome axis.Nature communications · 2026Article
- Article
- Parasitic plasmids are anchored to inactive regions of eukaryotic chromosomes through a nucleosome signal.The EMBO journal · 2025Article
- Absence of chromosome axis protein recruitment prevents meiotic recombination chromosome-wide in the budding yeastProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Meiosis in budding yeast.Genetics · 2023Article
- Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltii.PLoS genetics · 2023Article
- Karyotype engineering reveals spatio-temporal control of replication firing and gene contacts.Cell genomics · 2022Article
- Diffusion and distal linkages govern interchromosomal dynamics during meiotic prophase.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Review
- The 3D genome landscape: Diverse chromosomal interactions and their functional implications.Frontiers in cell and developmental biology · 2022Review
- Chromosome architecture and homologous recombination in meiosis.Frontiers in cell and developmental biology · 2022Review
- 3D chromatin structure changes during spermatogenesis and oogenesis.Computational and structural biotechnology journal · 2022Review
- Cohesin regulates homology search during recombinational DNA repair.Nature cell biology · 2021Article
- Stage-resolved Hi-C analyses reveal meiotic chromosome organizational features influencing homolog alignment.Nature communications · 2021Article
- Genome-wide variability in recombination activity is associated with meiotic chromatin organization.Genome research · 2021Article
- Getting there: understanding the chromosomal recruitment of the AAA+ ATPase Pch2/TRIP13 during meiosis.Current genetics · 2021Review
- How do small chromosomes know they are small? Maximizing meiotic break formation on the shortest yeast chromosomes.Current genetics · 2021Review
- Sir3 mediates long-range chromosome interactions in budding yeast.Genome research · 2021Article
- Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency.Science advances · 2021Article
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Authors and funding
13 authors.
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Abstract
In chromosome conformation capture experiments (Hi-C), the accuracy with which contacts are detected varies due to the uneven distribution of restriction sites along genomes. In addition, repeated sequences or homologous regions remain indistinguishable because of the ambiguities they introduce during the alignment of the sequencing reads. We addressed both limitations by designing and engineering 144 kb of a yeast chromosome with regularly spaced restriction sites (Syn-HiC design). In the Syn-HiC region, Hi-C signal-to-noise ratio is enhanced and can be used to measure the shape of an unbiased distribution of contact frequencies, allowing to propose a robust definition of a Hi-C experiment resolution. The redesigned region is also distinguishable from its native homologous counterpart in an otherwise isogenic diploid strain. As a proof of principle, we tracked homologous chromosomes during meiotic prophase in synchronized and pachytene-arrested cells and captured important features of their spatial reorganization, such as chromatin restructuration into arrays of Rec8-delimited loops, centromere declustering, individualization, and pairing. Overall, we illustrate the promises held by redesigning genomic regions to explore complex biological questions.
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